Four distinct phases of basket/stellate cell migration after entering their final destination (the molecular layer) in the developing cerebellum.
Four distinct phases of basket/stellate cell migration after entering their final destination (the molecular layer) in the developing cerebellum.
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DOI：
10.1016/j.ydbio.2009.05.575
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发表时间：
2009-08-15
影响因子：
2.7
通讯作者：
Komuro, Hitoshi
中科院分区：
文献类型：
--
作者：
Cameron, D. Bryant;Kasai, Kazue;Jiang, Yulan;Hu, Taofang;Saeki, Yoshinaga;Komuro, Hitoshi
关键词：
在成人小脑中，篮/星状细胞分散在整个 ML 中，但人们对细胞分散的过程知之甚少。为了确定星状/篮状细胞在 ML 内的分配，我们检查了它们在出生后早期小鼠小脑中的迁移。我们发现进入 ML 后，篮/星状细胞依次表现出四个不同的迁移阶段。首先，细胞从底部到顶部呈放射状迁移，同时表现出具有单一主导过程的跳跃运动（第一阶段）。其次，细胞在顶部转向并沿头尾方向切向迁移，偶尔会逆转迁移方向（第二阶段）。第三，细胞在 ML 内以显着降低的速度旋转和径向迁移，同时反复延伸和缩回主导过程（第三阶段）。第四，细胞在中间转向并以最慢的速度切向迁移，同时在完全撤回主导突起后延伸出几个类似树突的突起（第四阶段）。最后，细胞停止并完成迁移。这些结果表明，ML 中篮/星状细胞的分散是由外部引导线索、细胞间接触和内在程序以位置和时间依赖的方式精心策划的活动控制的。
In the adult cerebellum, basket/stellate cells are scattered throughout the ML, but little is known about the process underlying the cell dispersion. To determine the allocation of stellate/basket cells within the ML, we examined their migration in the early postnatal mouse cerebellum. We found that after entering the ML, basket/stellate cells sequentially exhibit four distinct phases of migration. First, the cells migrated radially from the bottom to the top while exhibiting saltatory movement with a single leading process (Phase I). Second, the cells turned at the top and migrated tangentially in a rostro-caudal direction, with an occasional reversal of the direction of migration (Phase II). Third, the cells turned and migrated radially within the ML at a significantly reduced speed while repeatedly extending and withdrawing the leading processes (Phase III). Fourth, the cells turned at the middle and migrated tangentially at their slowest speed, while extending several dendrite-like processes after having completely withdrawn the leading process (Phase IV). Finally, the cells stopped and completed their migration. These results suggest that the dispersion of basket/stellate cells in the ML is controlled by the orchestrated activity of external guidance cues, cell-cell contact and intrinsic programs in a position-and time-dependent manner.
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影响因子：
16.2
作者：
HEKMAT, A;KUNEMUND, V;SCHACHNER, M
通讯作者：
SCHACHNER, M
影响因子：
2.5
作者：
ALTMAN, J
通讯作者：
ALTMAN, J
影响因子：
2.5
作者：
ALTMAN, J
通讯作者：
ALTMAN, J
影响因子：
7.8
作者：
FUJITA, S
通讯作者：
FUJITA, S
影响因子：
3.4
作者：
HALLONET, MER;LEDOUARIN, NM
通讯作者：
LEDOUARIN, NM