Immunostimulatory activities of polysaccharide extract isolated from Curcuma longa.
Immunostimulatory activities of polysaccharide extract isolated from Curcuma longa.
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DOI：
10.1016/j.ijbiomac.2010.05.019
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发表时间：
2010-10-01
影响因子：
8.2
通讯作者：
Lau CB
中科院分区：
文献类型：
--
作者：
Yue GG;Chan BC;Hon PM;Kennelly EJ;Yeung SK;Cassileth BR;Fung KP;Leung PC;Lau CB
从姜黄中分离得到的姜黄素和倍半萜类化合物具有多种药理活性。在本研究中，使用人外周血单个核细胞（PBMC）研究CL热水提取物极性组分的免疫调节活性。我们的结果表明，热水提取物的高极性部分表现出刺激PBMC增殖的作用，如[甲基-3H]-胸苷掺入试验所示。在尝试分离负责活性的活性组分时，进一步用乙酸乙酯、正丁醇和乙醇逐步进行分配。细胞因子（TGF-β、TNF-α、GM-CSF、IL-1α、IL-5、IL-6、IL-8、IL-10和IL-13等）的产生与细胞凋亡密切相关。已经被富含多糖的部分调节，如ELISA和细胞因子蛋白阵列中所示。CD 14阳性染色的PBMC的比例通过该组分增加。用GC-MS和凝胶渗透色谱法测定了活性部位的单糖组成。首次证实姜黄多糖对PBMC有免疫刺激作用。研究结果揭示了姜黄粗提取物（含有姜黄素和多糖）作为癌症患者的辅助补充剂的潜在用途，这些患者的免疫活性在化疗期间受到抑制。
Several curcuminoids and sesquiterpenoids isolated from Curcuma longa (CL) have been shown to have many pharmacological activities. In the present study, the immunomodulatory activities of the polar fractions of CL hot water extracts were investigated using human peripheral blood mononuclear cells (PBMC). Our results showed that the high polarity fraction of the hot water extract exhibited stimulatory effects on PBMC proliferation as shown in [methyl-3H]-thymidine incorporation assay. In an attempt to isolate the active components responsible for the activities, further partition with ethyl-acetate, n-butanol and ethanol, progressively were performed. The cytokine productions (TGF-β, TNF-α, GM-CSF, IL-1α, IL-5, IL-6, IL-8, IL-10 and IL-13, etc.) have been modulated by a polysaccharide-enriched fraction as shown in ELISA and cytokine protein array. The proportion of CD14 positive stained PBMC was increased by such fraction. The composition of monosaccharide of the active fraction has been determined by GC-MS and gel permeation chromatography. The immunostimulatory effects of Curcuma longa polysaccharides on PBMC were shown for the first time. The findings revealed the potential use of Curcuma longa crude extract (containing curcuminoids and polysaccharides) as an adjuvant supplement for cancer patients, whose immune activities were suppressed during chemotherapies.
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影响因子：
4.3
作者：
Yue, Grace G. L.;Chan, Ben C. L.;Lau, Clara B. S.
通讯作者：
Lau, Clara B. S.
影响因子：
11.4
作者：
Lee, H. S.
通讯作者：
Lee, H. S.
影响因子：
11.2
作者：
Kunnumakkara, Ajaikumar B.;Guha, Sushovan;Aggarwal, Bharat B.
通讯作者：
Aggarwal, Bharat B.
影响因子：
1.7
作者：
GONDA, R;TAKEDA, K;TOMODA, M
通讯作者：
TOMODA, M
影响因子：
3.6
作者：
Aggarwal, S;Ichikawa, H;Aggarwal, BB
通讯作者：
Aggarwal, BB