Deoxynivalenol impairs hepatic and intestinal gene expression of selected oxidative stress, tight junction and inflammation proteins in broiler chickens, but addition of an adsorbing agent shifts the effects to the distal parts of the small intestine.
Deoxynivalenol impairs hepatic and intestinal gene expression of selected oxidative stress, tight junction and inflammation proteins in broiler chickens, but addition of an adsorbing agent shifts the effects to the distal parts of the small intestine.
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DOI：
10.1371/journal.pone.0069014
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发表时间：
2013
期刊：
影响因子：
3.7
通讯作者：
Croubels S
中科院分区：
文献类型：
--
作者：
Osselaere A;Santos R;Hautekiet V;De Backer P;Chiers K;Ducatelle R;Croubels S
肉鸡对脱氧雪腐镰刀菌烯醇有较强的抵抗力，因此临床症状很少见。然而，亚临床浓度的脱氧雪腐镰刀菌烯醇对肠道和肝脏的影响较少在分子水平上进行研究。在本研究中，我们研究了饲喂脱氧雪腐镰刀菌烯醇三周对肉鸡肠壁形态、肠道屏障功能和炎症的影响。此外，还对肝脏和肠道的氧化应激进行了评估。此外，还研究了粘土基真菌毒素吸附剂对上述不同方面的影响。结果表明，饲喂脱氧雪腐镰刀菌烯醇对肉鸡十二指肠和空肠的肠壁形态均有影响。QRT-PCR分析表明，脱氧雪腐镰刀菌烯醇对肠屏障的作用是非常特异的，因为只观察到空肠claudin 5的mRNA表达上调，而对claudin 1，zona occludens 1和2没有影响。加入一种吸附剂导致所有编码回肠肠屏障基因的上调。Toll样受体4和氧化应激的两个标志物(血红素氧合酶或HMOX和黄嘌呤氧化还原酶或XOR)的上调主要见于空肠，回肠次之，与吸附剂联合作用主要见于回肠。这些结果表明，吸附剂可能导致脱氧雪腐镰刀菌烯醇在更远端的小肠部分浓度较高。在肝脏中，由于接触DON，XOR表达上调。Hmox和HIF-1α(缺氧诱导因子1α)表达下调是由于饲喂DON，也是由于单独饲喂吸附剂或与DON联合饲喂。
Broiler chickens are rather resistant to deoxynivalenol and thus, clinical signs are rarely seen. However, effects of subclinical concentrations of deoxynivalenol on both the intestine and the liver are less frequently studied at the molecular level. During our study, we investigated the effects of three weeks of feeding deoxynivalenol on the gut wall morphology, intestinal barrier function and inflammation in broiler chickens. In addition, oxidative stress was evaluated in both the liver and intestine. Besides, the effect of a clay-based mycotoxin adsorbing agent on these different aspects was also studied. Our results show that feeding deoxynivalenol affects the gut wall morphology both in duodenum and jejenum of broiler chickens. A qRT-PCR analysis revealed that deoxynivalenol acts in a very specific way on the intestinal barrier, since only an up-regulation in mRNA expression of claudin 5 in jejunum was observed, while no effects were seen on claudin 1, zona occludens 1 and 2. Addition of an adsorbing agent resulted in an up-regulation of all the investigated genes coding for the intestinal barrier in the ileum. Up-regulation of Toll-like receptor 4 and two markers of oxidative stress (heme-oxigenase or HMOX and xanthine oxidoreductase or XOR) were mainly seen in the jejunum and to a lesser extent in the ileum in response to deoxynivalenol, while in combination with an adsorbing agent main effect was seen in the ileum. These results suggest that an adsorbing agent may lead to higher concentrations of deoxynivalenol in the more distal parts of the small intestine. In the liver, XOR was up-regulated due to DON exposure. HMOX and HIF-1α (hypoxia-inducible factor 1α) were down-regulated due to feeding DON but also due to feeding the adsorbing agent alone or in combination with DON.
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影响因子：
4.3
作者：
Frankic, T.;Pajk, T.;Salobir, J.
通讯作者：
Salobir, J.
DOI：
10.1083/jcb.141.7.1539
发表时间：
1998-06-29
期刊：
The Journal of cell biology
影响因子：
--
作者：
Furuse M;Fujita K;Hiiragi T;Fujimoto K;Tsukita S
通讯作者：
Tsukita S
影响因子：
4.4
作者：
Awad, W. A.;Ghareeb, K.;Boehm, J.
通讯作者：
Boehm, J.
影响因子：
4.4
作者：
Carro, M. D.;Falkenstein, E.;Klandorf, H.
通讯作者：
Klandorf, H.
影响因子：
5.6
作者：
Awad, Wageha A.;Hess, Michael;Zentek, Juergen
通讯作者：
Zentek, Juergen