SARS-CoV-2 RNA is enriched by orders of magnitude in primary settled solids relative to liquid wastewater at publicly owned treatment works.
SARS-CoV-2 RNA is enriched by orders of magnitude in primary settled solids relative to liquid wastewater at publicly owned treatment works.
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DOI：
10.1039/d1ew00826a
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发表时间：
2022-03-31
期刊：
影响因子：
--
通讯作者：
Boehm AB
中科院分区：
文献类型：
--
作者：
Kim S;Kennedy LC;Wolfe MK;Criddle CS;Duong DH;Topol A;White BJ;Kantor RS;Nelson KL;Steele JA;Langlois K;Griffith JF;Zimmer-Faust AG;McLellan SL;Schussman MK;Ammerman M;Wigginton KR;Bakker KM;Boehm AB
基于水的流行病学在全世界范围内受到关注，用于检测废水中的SARS-CoV-2 RNA，以补充临床检测。原废水由悬浮在液体中的小颗粒或固体组成。已经开发了测量废水的液体和固体部分中的SARS-CoV-2 RNA的方法，一些研究报告固体部分中的浓度较高。为了进一步研究这种关系，六个实验室合作进行了一项研究，在五个公有的处理厂（POTW），从初级澄清池和原废水进水样品获得的初级沉降固体收集和定量的SARS冠状病毒-2 RNA。参与实验室使用各自的方法处理沉降固体和进水样品，并根据采集日期进行回顾性配对。SARS-CoV-2 RNA浓度，在质量当量的基础上，在沉淀的固体比在进水中高约三个数量级。在匹配的沉降固体和进水浓度呈正相关，显着相关，在所有五个POTW。沉降固体和流入物中的RNA浓度与下水道中的COVID-19发病率相关，因此代表了疾病的发生;沉降固体方法似乎在所有POTW的SARS-CoV-2 RNA浓度测量值和发病率之间产生了可比的关系。沉降固体和进水的方法表现出可比的灵敏度，N基因检测频率，并计算出经验发病率下限。SARS-CoV-2 RNA的沉降固体分析具有使用较少样品体积以实现与流入物方法相似的灵敏度的优点。我们比较了SARS-CoV-2 RNA浓度的主要沉降固体和原废水样品匹配的日期，以调查两个矩阵之间的关系。
Wastewater-based epidemiology has gained attention throughout the world for detection of SARS-CoV-2 RNA in wastewater to supplement clinical testing. Raw wastewater consists of small particles, or solids, suspended in liquid. Methods have been developed to measure SARS-CoV-2 RNA in the liquid and the solid fraction of wastewater, with some studies reporting higher concentrations in the solid fraction. To investigate this relationship further, six laboratories collaborated to conduct a study across five publicly owned treatment works (POTWs) where both primary settled solids obtained from primary clarifiers and raw wastewater influent samples were collected and quantified for SARS-CoV-2 RNA. Settled solids and influent samples were processed by participating laboratories using their respective methods and retrospectively paired based on date of collection. SARS-CoV-2 RNA concentrations, on a mass equivalent basis, were higher in settled solids than in influent by approximately three orders of magnitude. Concentrations in matched settled solids and influent were positively and significantly correlated at all five POTWs. RNA concentrations in both settled solids and influent were correlated to COVID-19 incidence rates in the sewersheds and thus representative of disease occurrence; the settled solids methods appeared to produce a comparable relationship between SARS-CoV-2 RNA concentration measurements and incidence rates across all POTWs. Settled solids and influent methods showed comparable sensitivity, N gene detection frequency, and calculated empirical incidence rate lower limits. Analysis of settled solids for SARS-CoV-2 RNA has the advantage of using less sample volume to achieve similar sensitivity to influent methods. We compared SARS-CoV-2 RNA concentrations in primary settled solids and raw wastewater samples matched in date to investigate the relationship between the two matrices.
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影响因子：
3.4
作者：
Flood MT;D'Souza N;Rose JB;Aw TG
通讯作者：
Aw TG
影响因子：
1.6
作者：
Opel, Kerry L.;Chung, Denise;McCord, Bruce R.
通讯作者：
McCord, Bruce R.
DOI：
10.1021/acsestwater.1c00160
发表时间：
2021-07-09
期刊：
ACS ES&T WATER
影响因子：
--
作者：
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通讯作者：
McLellan, Sandra L.
影响因子：
11.4
作者：
Graham, Katherine E.;Loeb, Stephanie K.;Boehm, Alexandria B.
通讯作者：
Boehm, Alexandria B.
影响因子：
6.4
作者：
Karthikeyan S;Ronquillo N;Belda-Ferre P;Alvarado D;Javidi T;Longhurst CA;Knight R
通讯作者：
Knight R