Effects of Dietary Different Doses of Copper and High Fructose Feeding on Rat Fecal Metabolome.
Effects of Dietary Different Doses of Copper and High Fructose Feeding on Rat Fecal Metabolome.
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DOI：
10.1021/acs.jproteome.5b00596
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发表时间：
2015-09-04
影响因子：
4.4
通讯作者：
Zhang X
中科院分区：
文献类型：
--
作者：
Wei X;Song M;Yin X;Schuschke DA;Koo I;McClain CJ;Zhang X
肠道微生物群在非酒精性脂肪性肝病（NAFLD）的发病机制中发挥着关键作用。果糖摄入量增加和铜摄入不足是 NAFLD 发展的两个关键危险因素。为了深入了解肠道微生物群的作用，通过全面的二维气相色谱飞行时间质谱 (GC × GC-TOF MS) 分析了暴露于不同铜含量（含或不含高果糖摄入）的大鼠 4 周的粪便代谢物。同时，通过组织学和甘油三酯测定评估肝组织。我们的数据显示，高果糖喂养导致边缘铜缺乏大鼠和铜补充大鼠明显的肝脏脂肪变性。在检测到的 38 种代谢物中，组间丰度存在显着变化，无论铜水平如何，短链脂肪酸都会随着果糖摄入过多而显着减少。仅由细菌产生的 C15:0 和 C17:0 长链脂肪酸会因高铜含量或高果糖摄入而增加。此外，粪便尿素和苹果酸的增加与肝脏脂肪积累的增加平行。总的来说，对大鼠粪便样本的 GC × GC-TOF MS 分析揭示了与饮食中高果糖和铜水平相关的独特粪便代谢组谱，其中一些代谢物可能作为果糖诱导的 NAFLD 的潜在非侵入性生物标志物。
The gut microbiota plays a critical role in the pathogenesis of nonalcoholic fatty liver disease (NAFLD). Increased fructose consumption and inadequate copper intake are two critical risk factors in the development of NAFLD. To gain insight into the role of gut microbiota, fecal metabolites, obtained from rats exposed to different dietary levels of copper with and without high fructose intake for 4 weeks, were analyzed by comprehensive two-dimensional gas chromatography time-of-flight mass spectrometry (GC × GC-TOF MS). In parallel, liver tissues were assessed by histology and triglyceride assay. Our data showed that high fructose feeding led to obvious hepatic steatosis in both marginal copper deficient rats and copper supplementation rats. Among the 38 metabolites detected with significant abundance alteration between groups, short chain fatty acids were markedly decreased with excessive fructose intake irrespective of copper levels. C15:0 and C17:0 long chain fatty acids, produced only by bacteria, were increased by either high copper level or high fructose intake. In addition, increased fecal urea and malic acid paralleled the increased hepatic fat accumulation. Collectively, GC × GC-TOF MS analysis of rat fecal samples revealed distinct fecal metabolome profiles associated with the dietary high fructose and copper level, with some metabolites possibly serving as potential noninvasive biomarkers of fructose induced-NAFLD.
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影响因子：
25.7
作者：
Ouyang, Xiaosen;Cirillo, Pietro;Abdelmalek, Manal F.
通讯作者：
Abdelmalek, Manal F.
影响因子：
25.7
作者：
Bergheim, Ina;Weber, Synia;Bischoff, Stephan C.
通讯作者：
Bischoff, Stephan C.
乳糖乳杆菌GG对小鼠酒精脂肪肝病的影响的肝和粪便代谢组学分析。
影响因子：
4.4
作者：
Shi X;Wei X;Yin X;Wang Y;Zhang M;Zhao C;Zhao H;McClain CJ;Feng W;Zhang X
通讯作者：
Zhang X
影响因子：
16.2
作者：
Bray GA
通讯作者：
Bray GA
影响因子：
15.9
作者：
Stanhope, Kimber L.;Schwarz, Jean Marc;Havel, Peter J.
通讯作者：
Havel, Peter J.