Subcellular partitioning of protein kinase activity revealed by functional kinome profiling.
Subcellular partitioning of protein kinase activity revealed by functional kinome profiling.
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DOI：
10.1038/s41598-022-21026-5
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发表时间：
2022-10-15
影响因子：
4.6
通讯作者：
中科院分区：
文献类型：
--
作者：
蛋白激酶和它们的底物形成跨亚细胞区室分配的信号网络，以促进关键的生物过程。虽然许多单个激酶的亚细胞作用已被阐明，但缺乏对突触亚激酶组的全面评估。此外，大多数激酶的研究集中在转录物、蛋白质和/或磷蛋白表达水平上，提供了蛋白激酶活性的间接测量。先前的工作表明，基因表达水平不是蛋白质功能的良好预测因子。因此，我们评估了全球丝氨酸/苏氨酸蛋白激酶的活性分布在突触体，核，和胞质组分从大鼠额叶皮质匀浆肽阵列。级分之间的比较表明总体蛋白激酶活性的差异。上游激酶分析揭示了一系列同源激酶，这些激酶与核组分相比富集在突触体组分中。我们确定了许多激酶的突触部分以前牵连在这个隔间，同时也确定了其他激酶很少或没有证据的突触定位。我们的研究结果表明，评估亚细胞组分与肽活性阵列的可行性，以及建议与建立和新的激酶的隔室比活性配置文件。
Protein kinases and their substrates form signaling networks partitioned across subcellular compartments to facilitate critical biological processes. While the subcellular roles of many individual kinases have been elucidated, a comprehensive assessment of the synaptic subkinome is lacking. Further, most studies of kinases focus on transcript, protein, and/or phospho-protein expression levels, providing an indirect measure of protein kinase activity. Prior work suggests that gene expression levels are not a good predictor of protein function. Thus, we assessed global serine/threonine protein kinase activity profiles in synaptosomal, nuclear, and cytosolic fractions from rat frontal cortex homogenate using peptide arrays. Comparisons made between fractions demonstrated differences in overall protein kinase activity. Upstream kinase analysis revealed a list of cognate kinases that were enriched in the synaptosomal fraction compared to the nuclear fraction. We identified many kinases in the synaptic fraction previously implicated in this compartment, while also identifying other kinases with little or no evidence for synaptic localization. Our results show the feasibility of assessing subcellular fractions with peptide activity arrays, as well as suggesting compartment specific activity profiles associated with established and novel kinases.
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影响因子：
16.2
作者：
Kim, Sung Hyun;Ryan, Timothy A.
通讯作者：
Ryan, Timothy A.
DOI：
10.1126/science.1190287
发表时间：
2010-08-20
期刊：
Science (New York, N.Y.)
影响因子：
--
作者：
Li N;Lee B;Liu RJ;Banasr M;Dwyer JM;Iwata M;Li XY;Aghajanian G;Duman RS
通讯作者：
Duman RS
影响因子：
5.2
作者：
Krayem, Mohamad;Aftimos, Philippe;Ghanem, Ghanem E.
通讯作者：
Ghanem, Ghanem E.
影响因子：
3.5
作者：
Appuhamy, J. A. D. R. N.;Nayananjalie, W. A.;Hanigan, M. D.
通讯作者：
Hanigan, M. D.
DOI：
10.1002/cpz1.90
发表时间：
2021-03
期刊：
Current protocols
影响因子：
--
作者：
Xie Z;Bailey A;Kuleshov MV;Clarke DJB;Evangelista JE;Jenkins SL;Lachmann A;Wojciechowicz ML;Kropiwnicki E;Jagodnik KM;Jeon M;Ma'ayan A
通讯作者：
Ma'ayan A