Sentinel Cards Provide Practical SARS-CoV-2 Monitoring in School Settings.
Sentinel Cards Provide Practical SARS-CoV-2 Monitoring in School Settings.
复制标题
DOI：
10.1128/msystems.00109-22
复制
发表时间：
2022-08-30
期刊：
影响因子：
6.4
通讯作者：
中科院分区：
文献类型：
--
作者：
帮助学生安全地返回亲自学习的一个有希望的方法是通过应用哨兵卡对室内SARS-CoV-2痕迹进行精确的高分辨率环境监测。由于SARS-CoV-2 RNA可以在几种室内表面材料上持续长达一周，因此需要提高时间分辨率，以确定连续的表面阳性是否来自新的感染事件或继续报告过去的事件。采样后清洁哨兵卡可提供所需的分辨率，但可能会干扰检测性能。我们在三种不同的病毒载量下测试了三种清洁溶液（BZK湿巾、湿巾、RNase Away）的效果：“高”（4 × 104 GE/mL）、“中”（1 × 104 GE/mL）和“低”（2.5 × 103 GE/mL）。选择作为阳性对照的RNase Away是对所有三种病毒载量最有效的清洁溶液。发现湿巾在中等病毒载量条件下比BZK湿巾更有效。使用所有三种清洁溶液可轻松重置低病毒载量条件。这些发现将使在室内环境中进行SARS-CoV-2的时间监测成为可能，因为室内环境中病毒的传播风险很高，并且出于隐私或合规原因需要避免个人水平的采样。重要性由于引起COVID-19的SARS-CoV-2病毒在表面上持续存在，因此从表面采集的拭子检测可用作监测工具。这种方法在学校环境中特别有价值，因为在学校环境中，通过从教室中提取单个样本可以消除成本和隐私问题。然而，病毒在表面样本上持续存在数天至数周，因此无法判断连续几天的阳性检测事件是持续信号还是新的传染病例，因此无法判断阳性个体是否已被成功从教室中清除。我们比较了几种清洁“哨兵卡”的方法，以表明这种方法可以用于识别新的SARS-CoV-2信号。这些结果对于确定如何监测教室和其他室内环境中的SARS-CoV-2病毒非常重要。
A promising approach to help students safely return to in person learning is through the application of sentinel cards for accurate high resolution environmental monitoring of SARS-CoV-2 traces indoors. Because SARS-CoV-2 RNA can persist for up to a week on several indoor surface materials, there is a need for increased temporal resolution to determine whether consecutive surface positives arise from new infection events or continue to report past events. Cleaning sentinel cards after sampling would provide the needed resolution but might interfere with assay performance. We tested the effect of three cleaning solutions (BZK wipes, Wet Wipes, RNase Away) at three different viral loads: “high” (4 × 104 GE/mL), “medium” (1 × 104 GE/mL), and “low” (2.5 × 103 GE/mL). RNase Away, chosen as a positive control, was the most effective cleaning solution on all three viral loads. Wet Wipes were found to be more effective than BZK wipes in the medium viral load condition. The low viral load condition was easily reset with all three cleaning solutions. These findings will enable temporal SARS-CoV-2 monitoring in indoor environments where transmission risk of the virus is high and the need to avoid individual-level sampling for privacy or compliance reasons exists. IMPORTANCE Because SARS-CoV-2, the virus that causes COVID-19, persists on surfaces, testing swabs taken from surfaces is useful as a monitoring tool. This approach is especially valuable in school settings, where there are cost and privacy concerns that are eliminated by taking a single sample from a classroom. However, the virus persists for days to weeks on surface samples, so it is impossible to tell whether positive detection events on consecutive days are a persistent signal or new infectious cases and therefore whether the positive individuals have been successfully removed from the classroom. We compare several methods for cleaning “sentinel cards” to show that this approach can be used to identify new SARS-CoV-2 signals day to day. The results are important for determining how to monitor classrooms and other indoor environments for SARS-CoV-2 virus.
登录
查看更多内容
影响因子：
--
作者：
Quigley, Maire F;Almeida, Jorge R;Price, David A;Douek, Daniel C
通讯作者：
Douek, Daniel C
影响因子：
6.4
作者：
Karthikeyan S;Nguyen A;McDonald D;Zong Y;Ronquillo N;Ren J;Zou J;Farmer S;Humphrey G;Henderson D;Javidi T;Messer K;Anderson C;Schooley R;Martin NK;Knight R
通讯作者：
Knight R
室内灰尘作为矩阵，用于监视COVID-19。
影响因子：
6.4
作者：
Renninger N;Nastasi N;Bope A;Cochran SJ;Haines SR;Balasubrahmaniam N;Stuart K;Bivins A;Bibby K;Hull NM;Dannemiller KC
通讯作者：
Dannemiller KC
DOI：
10.1016/j.jhin.2020.11.023
发表时间：
2021-03
期刊：
The Journal of hospital infection
影响因子：
--
作者：
Ogilvie BH;Solis-Leal A;Lopez JB;Poole BD;Robison RA;Berges BK
通讯作者：
Berges BK
影响因子：
5.7
作者：
Kwok CS;Dashti M;Tafuro J;Nasiri M;Muntean EA;Wong N;Kemp T;Hills G;Mallen CD
通讯作者：
Mallen CD