A broadly tuned odorant receptor in neurons of trichoid sensilla in locust, Locusta migratoria.
A broadly tuned odorant receptor in neurons of trichoid sensilla in locust, Locusta migratoria.
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蝗虫毛状感觉器神经元中广泛调节的气味受体
DOI：
10.1016/j.ibmb.2016.10.008
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发表时间：
2016-12
影响因子：
3.8
通讯作者：
Zhang L
中科院分区：
文献类型：
--
作者：
You Y;Smith DP;Lv M;Zhang L
昆虫已经进化出复杂的嗅觉接收系统来感知外部化学信号。化学感觉神经元膜上的气味受体(ORs)被认为是感知外源性化学线索的关键分子。不同种类昆虫的ORS是不同的，应该将一个物种调整到与其生存相关的特定信号化学物质。直翅目昆虫蝗虫(Locusta Migratoria)是一种典型的半代谢昆虫。虽然在基因组测序实验中已经发现了许多蝗虫OR基因，但对蝗虫OR基因的功能知之甚少。本文用原位杂交的方法，将蝗虫OR，LmiOR3定位于毛状体感受器内的神经元。在缺乏内源性受体Or67d的果蝇毛状嗅觉神经元(AT1)中以转基因的形式表达LmiOR3，并建立了该受体的嗅觉调节曲线和剂量-反应曲线。结果表明，LmiOR3使神经元对酮、酯和杂环化合物敏感，表明LmiOR3是一种广调受体。LmiOR3是第一个直翅目气味受体，已在果蝇AT1系统中进行了功能分析。这项工作证明了果蝇AT1系统用于蝗虫气味受体功能分析的有效性，并表明LmiOR3可能参与检测食物气味或蝗虫体内挥发物，这可能有助于我们开发防治蝗虫的新方法。气味受体LmiOR3在蝗虫毛状体感受器中表达，并在转基因果蝇触角毛状体感受器(AT1)中广泛调节C7-C10酮、C5-C8酯和C5-C9异环化合物的气味。
Insects have evolved sophisticated olfactory reception systems to sense exogenous chemical signals. Odorant receptors (ORs) on the membrane of chemosensory neurons are believed to be key molecules in sensing exogenous chemical cues. ORs in different species of insects are diverse and should tune a species to its own specific semiochemicals relevant to their survival. The orthopteran insect, locust (Locusta migratoria), is a model hemimetabolous insect. There is very limited knowledge on the functions of locust ORs although many locust OR genes have been identified in genomic sequencing experiments. In this paper, a locust OR, LmigOR3 was localized to neurons housed in trichoid sensilla by in situ hybridization. LmigOR3 was expressed as a transgene in Drosophila trichoid olfactory neurons (aT1) lacking the endogenous receptor Or67d and the olfactory tuning curve and dose-response curves were established for this locust receptor. The results show that LmigOR3 sensitizes neurons to ketones, esters and heterocyclic compounds, indicating that LmigOR3 is a broadly tuned receptor. LmigOR3 is the first odorant receptor from Orthoptera that has been functionally analyzed in the Drosophila aT1 system. This work demonstrates the utility of the Drosophila aT1 system for functional analysis of locust odorant receptors and suggests that LmigOR3 may be involved in detecting food odorants, or perhaps locust body volatiles that may help us to develop new control methods for locusts. An odorant receptor, LmigOR3 is expressed in trichoid sensilla of locust (Locusta migratoria) and broadly tunes to odorants of C7–C10 ketones, C5–C8 esters and C5–C9 heterocyclics in antennal trichoid sensilla (aT1) of transgenic Drosophila.
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影响因子：
3.8
作者：
Smart, Renee;Kiely, Aidan;Warr, Coral G.
通讯作者：
Warr, Coral G.
DOI：
10.1073/pnas.1003881107
发表时间：
2010-05-18
影响因子：
11.1
作者：
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通讯作者：
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影响因子：
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作者：
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通讯作者：
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影响因子：
64.8
作者：
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通讯作者：
Dickson, Barry J.
影响因子：
2.2
作者：
Cui, Xiaojie;Wu, Caihong;Zhang, Long
通讯作者：
Zhang, Long