The potential of encapsulating "raw materials" in 3D osteochondral gradient scaffolds.
The potential of encapsulating "raw materials" in 3D osteochondral gradient scaffolds.
复制标题
DOI：
10.1002/bit.25145
复制
发表时间：
2014-04
影响因子：
3.8
通讯作者：
Detamore, Michael S.
中科院分区：
文献类型：
--
作者：
Mohan, Neethu;Gupta, Vineet;Sridharan, BanuPriya;Sutherland, Amanda;Detamore, Michael S.
关键词：
材料组成和生物活性信号具有连续梯度的支架使界面上的性能能够平稳过渡。三维支架中的硫酸软骨素(CS)和生物活性玻璃(BG)等成分可作为合成新的细胞外基质(ECM)的原料，并有可能完全或部分取代昂贵的生长因子。我们假设，具有ECM成分梯度的支架将使工程构造具有优异的性能。原材料的包埋改变了支架的外观、结构、孔隙率和降解。他们允许支架在培养过程中更好地保留其3D结构，并为培养中的细胞提供缓冲效应。在种植大鼠间充质干细胞后，有几次发现含有原料(CS或BG)的支架的糖胺聚糖(GAG)、胶原或钙的含量高于含有转化生长因子-β3或骨形态发生蛋白-2的支架。同样值得注意的是，CS和转化生长因子-β-3的组合增加了II型胶原的分泌。此外，种植在含有相反梯度的CS/转化生长因子-β3和BG/BMP-2的支架中的细胞在组织特异性ECM的分泌方面产生了明显的区域性差异。研究表明，原材料有可能为细胞创造良好的微环境；与昂贵的生长因子相比，它们可以显著促进某些细胞外基质(ECM)成分的合成；无论是单独使用还是与生长因子结合使用，它们都可以促进组织特异性基质蛋白的分泌。原材料是很有希望的候选材料，既可以用来取代增长因素，也可以与增长因素结合使用。用原材料代替生长因子的成功可能会对更低的成本和更快的监管批准，以更快地将再生医学产品转化为临床产生深远的影响。
Scaffolds with continuous gradients in material composition and bioactive signals enable a smooth transition of properties at the interface. Components like chondroitin sulfate (CS) and bioactive glass (BG) in 3D scaffolds may serve as “raw materials” for synthesis of new extracellular matrix (ECM), and may have the potential to completely or partially replace expensive growth factors. We hypothesized that scaffolds with gradients of ECM components would enable superior performance of engineered constructs. Raw material encapsulation altered the appearance, structure, porosity, and degradation of the scaffolds. They allowed the scaffolds to better retain their 3D structure during culture and provided a buffering effect to the cells in culture. Following seeding of rat mesenchymal stem cells, there were several instances where glycosaminoglycan (GAG), collagen, or calcium contents were higher with the scaffolds containing raw materials (CS or BG) than with those containing transforming growth factor (TGF)-β3 or bone morphogenetic protein (BMP)-2. It was also noteworthy that a combination of both CS and TGF-β3 increased the secretion of collagen type II. Moreover, cells seeded in scaffolds containing opposing gradients of CS/TGF-β3 and BG/BMP-2 produced clear regional variations in the secretion of tissue-specific ECM. The study demonstrated raw materials have the potential to create a favorable microenvironment for cells; they can significantly enhance the synthesis of certain extracellular matrix (ECM) components when compared to expensive growth factors; either alone or in combination with growth factors they can enhance the secretion of tissue specific matrix proteins. Raw materials are promising candidates that can be used to either replace or be used in combination with growth factors. Success with raw materials in lieu of growth factors could have profound implications in terms of lower cost and faster regulatory approval for more rapid translation of regenerative medicine products to the clinic.
登录
查看更多内容
影响因子：
14
作者：
Chen, Yen-Lin;Lee, Hsiao-Ping;Hu, Yu-Chen
通讯作者：
Hu, Yu-Chen
影响因子：
9.7
作者：
Rahaman, Mohamed N.;Day, Delbert E.;Bal, B. Sonny;Fu, Qiang;Jung, Steven B.;Bonewald, Lynda F.;Tomsia, Antoni P.
通讯作者：
Tomsia, Antoni P.
影响因子：
14
作者：
Kato, D;Takeuchi, M;Kunitake, M
通讯作者：
Kunitake, M
DOI：
10.1007/s10856-009-3889-8
发表时间：
2010-02-01
影响因子：
3.7
作者：
Wu, Chun-Hsien;Ko, Chih-Sheng;Chu, I-Ming
通讯作者：
Chu, I-Ming
影响因子：
14
作者：
Newman, KD;McBurney, MW
通讯作者：
McBurney, MW