Hippocampal serotonin-1A receptor function in a mouse model of anxiety induced by long-term voluntary wheel running.
Hippocampal serotonin-1A receptor function in a mouse model of anxiety induced by long-term voluntary wheel running.
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DOI：
10.1002/syn.21666
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发表时间：
2013-10
期刊：
影响因子：
2.3
通讯作者：
Hensler, Julie G.
中科院分区：
文献类型：
--
作者：
Fuss, Johannes;Vogt, Miriam A.;Weber, Klaus-Josef;Burke, Teresa F.;Gass, Peter;Hensler, Julie G.
我们最近证明，在C57/BL6小鼠中，长期自主车轮跑是引起焦虑的，而海马区的局部照射可以阻止车轮跑引起的焦虑样行为的增加以及海马区的神经生物学变化。有证据支持海马5-HT1a受体在焦虑中的作用。因此，我们在焦虑的小鼠模型中研究了5-HT1a受体在海马区的结合和功能。通过放射自显影和激动剂刺激的[~(35)S]GTPγS与G蛋白结合的放射自显影测定，4周的自主车轮运动导致了海马区特异性的5-HT1a受体结合部位和功能的改变。在背侧CA1区，5-HT1a受体的结合和功能不受车轮转动和照射的影响。在背齿状回和CA2/3区，5-HT1a受体功能不仅受到跑步的影响，而且受到辐射的影响。在锥体腹侧层，车轮运动导致5-HT1a受体功能下降，这一作用可被辐射所阻止。前额内侧皮质5-HT1a受体、中缝背核和中缝正中核的5-HT1a受体不受照射和滚轮运动的影响。我们的数据表明，腹侧锥体层5-HT1a受体功能的下调可能在车轮转动诱导的焦虑样行为中起作用。
We have recently demonstrated that in C57/Bl6 mice long term voluntary wheel running is anxiogenic, and focal hippocampal irradiation prevents the increase in anxiety-like behaviors as well as neurobiological changes in the hippocampus induced by wheel running. Evidence supports a role of hippocampal 5-HT1A receptors in anxiety. Therefore, we investigated hippocampal binding and function of 5-HT1A receptors in this mouse model of anxiety. Four weeks of voluntary wheel running resulted in hippocampal subregion-specific changes in 5-HT1A receptor binding sites and function, as measured by autoradiography of [3H]8-OH-DPAT binding and agonist-stimulated binding of [35S]GTPγS to G proteins, respectively. In the dorsal CA1 region, 5-HT1A receptor binding and function were not altered by wheel running or irradiation. In the dorsal dentate gyrus and CA2/3 region, 5-HT1A receptor function was decreased by running, but also by irradiation. In the ventral pyramidal layer, wheel running resulted in a decrease of 5-HT1A receptor function, which was prevented by irradiation. Neither irradiation nor wheel running affected 5-HT1A receptors in medial prefrontal cortex, or in the dorsal or median raphe nuclei. Our data indicate that down-regulation of 5-HT1A receptor function in ventral pyramidal layer may play a role in anxiety-like behavior induced by wheel running.
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