ATH1 and KNAT2 proteins act together in regulation of plant inflorescence architecture.
ATH1 and KNAT2 proteins act together in regulation of plant inflorescence architecture.
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ATH1 和 KNAT2 蛋白共同作用调节植物花序结构
DOI：
10.1093/jxb/err376
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发表时间：
2012-02
影响因子：
6.9
通讯作者：
Xu L
中科院分区：
文献类型：
--
作者：
Li Y;Pi L;Huang H;Xu L
开花植物的花序是一种高度组织化的结构，不仅有助于植物的生殖过程，而且构成整个植物形态的重要组成部分。以前的研究表明，类KNOTTED 1同源盒（KNOX）基因BREVIPEDICELLUS（BP或KNAT 1），KNAT 2和KNAT 6在花序结构中起着重要作用。已知花梗形态对花序结构有很大贡献，BP负调节KNAT 2和KNAT 6以确保花梗具有正常的向上指向的方向。这些发现表明，一个遗传网络存在于控制花梗的方向，但如何在发展过程中这个网络的功能仍然是难以捉摸的。本文报道拟南芥同源框基因1（ATH 1）是一类KNOX网络中参与花梗定向调控的新成员，属于BELL 1-like homeodomain基因家族。在对isoginchaku-2D的抑制子的遗传筛选中，获得了一个抑制子突变体，该抑制子突变体是一种功能获得性不对称叶2突变体，显示向下指向的花梗。对该突变体的表征显示，该突变对应于ATH 1。遗传分析表明ATH 1主要作用于KNAT 2通路。酵母双杂交和双分子荧光互补分析表明，ATH 1物理相互作用与KNAT 2。数据表明，ATH 1-KNAT 2复合物与KNAT 6冗余地起作用，这两者在花梗发育期间都受BP负调控。
The inflorescence of flowering plants is a highly organized structure, not only contributing to plant reproductive processes, but also constituting an important part of the entire plant morphology. Previous studies have revealed that the class-I KNOTTED1-like homeobox (KNOX) genes BREVIPEDICELLUS (BP or KNAT1), KNAT2, and KNAT6 play essential roles in inflorescence architecture. Pedicel morphology is known to contribute greatly to inflorescence architecture, and BP negatively regulates KNAT2 and KNAT6 to ensure that pedicels have a normal upward-pointing orientation. These findings indicate that a genetic network exists in controlling pedicel orientation, but how this network functions in the developmental process remains elusive. Here it is reported that the ARABIDOPSIS THALIANA HOMEOBOX GENE1 (ATH1) gene, which belongs to the BELL1-like homeodomain gene family, is a new member participating in regulating pedicel orientation in the class-I KNOX network. In a genetic screening for suppressors of isoginchaku-2D, a gain-of-function ASYMMETRIC LEAVES2 mutant that displays downward-pointing pedicels, a suppressor mutant was obtained. Characterization of this mutant revealed that the mutation corresponds to ATH1. Genetic analysis indicated that ATH1 acts mainly in the KNAT2 pathway. Yeast two-hybrid and bimolecular fluorescence complementation assays demonstrated that ATH1 physically interacts with KNAT2. The data indicate that the ATH1–KNAT2 complex acts redundantly with KNAT6, both of which are negatively regulated by BP during pedicel development.
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影响因子：
14.9
作者：
Cole, M;Nolte, C;Werr, W
通讯作者：
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作者：
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