Phospholipid-grafted PLLA electrospun micro/nanofibers immobilized with small extracellular vesicles from rat adipose mesenchymal stem cells promote wound healing in diabetic rats.
Phospholipid-grafted PLLA electrospun micro/nanofibers immobilized with small extracellular vesicles from rat adipose mesenchymal stem cells promote wound healing in diabetic rats.
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磷脂接枝 PLLA 电纺微/纳米纤维固定有来自大鼠脂肪间充质干细胞的小细胞外囊泡，可促进糖尿病大鼠的伤口愈合。
DOI：
10.1093/rb/rbac071
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发表时间：
2022
影响因子：
6.7
通讯作者：
Fu, Xiaoling
中科院分区：
文献类型：
--
作者：
Li, Jing;Yan, Shunshun;Han, Weiju;Dong, Zixuan;Li, Junliang;Wu, Qi;Fu, Xiaoling
Small extracellular vesicles (sEVs) derived from mesenchymal stem cells (MSCs) can deliver a variety of bioactive factors to create a favorable local microenvironment, thereby holding huge potential in chronic wound repair. However, free sEVs administrated intravenously or locally are usually cleared rapidly, resulting in an insufficient duration of the efficacy. Thus, strategies that enable optimized retention and release profiles of sEVs at wound sites are desirable. Herein, we fabricated novel functional phosphoethanolamine phospholipid-grafted poly-l-lactic acid micro/nanofibers (DSPE-PLLA) to carry and retain sEVs from rat adipose MSCs, enabling the slow local release of sEVs. Our results showed that sEVs@DSPE-PLLA promoted the proliferation, migration and gene expression (Col I, Col III, TGF-β, α-SMA, HIF-1α) of fibroblasts. It also promoted keratinocyte proliferation. In addition, sEVs@DSPE-PLLA helped polarize macrophages toward the M2 phenotype by increasing the expression of anti-inflammatory genes (Arginase 1, CD 206, IL-10) and inhibiting the expression of pro-inflammatory genes (IL-1β, TNF-α). Further in vivo study in diabetic rat models showed that sEVs@DSPE-PLLA improved the wound-healing process by alleviating the inflammatory responses, stimulating cell proliferation, collagen deposition and angiogenesis. These results highlight the potential of using DSPE-grafted scaffolds for extracellular vesicle immobilization and suggest sEVs@DSPE-PLLA micro/nanofibers as promising functional wound dressings for diabetic wounds.
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DOI：
10.1111/bjd.13954
发表时间：
2015-08
期刊：
The British journal of dermatology
影响因子：
--
作者：
Martin P;Nunan R
通讯作者：
Nunan R
影响因子：
6
作者：
Lo Sicco C;Reverberi D;Balbi C;Ulivi V;Principi E;Pascucci L;Becherini P;Bosco MC;Varesio L;Franzin C;Pozzobon M;Cancedda R;Tasso R
通讯作者：
Tasso R
影响因子：
0.9
作者：
Bauer, Stephen M;Bauer, Richard J;Velazquez, Omaida C
通讯作者：
Velazquez, Omaida C
与富含醌的聚多巴胺涂层相关的增强血液相容性和内皮细胞增殖的体外研究
影响因子：
9.5
作者：
Luo, Rifang;Tang, Linlin;Huang, Nan
通讯作者：
Huang, Nan
影响因子：
17.1
作者：
Henriques-Antunes, Helena;Cardoso, Renato M. S.;Ferreira, Lino
通讯作者：
Ferreira, Lino