Absence of xenotropic murine leukaemia virus-related virus in UK patients with chronic fatigue syndrome.
Absence of xenotropic murine leukaemia virus-related virus in UK patients with chronic fatigue syndrome.
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DOI：
10.1186/1742-4690-7-10
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发表时间：
2010-02-15
期刊：
影响因子：
3.3
通讯作者：
Bishop KN
中科院分区：
文献类型：
--
作者：
Groom HC;Boucherit VC;Makinson K;Randal E;Baptista S;Hagan S;Gow JW;Mattes FM;Breuer J;Kerr JR;Stoye JP;Bishop KN
最近有报道称，在67%的慢性疲劳综合征患者中检测到逆转录病毒，异嗜性小鼠白血病病毒相关病毒（XMRV）。我们研究了来自两个英国队列的170个慢性疲劳综合征患者样本和395个对照样本，通过使用定量PCR（检测限<16个病毒拷贝）寻找病毒核酸的存在或使用病毒中和试验寻找血清学反应的存在，以寻找XMRV感染的证据。我们没有通过PCR在任何样品中鉴定出XMRV DNA（0/299）。一些血清样品显示XMRV中和活性（26/565），但这些阳性血清中只有一份来自CFS患者。大多数阳性血清还能够中和用其他病毒（包括水泡性口炎病毒）的包膜蛋白假型化的MLV颗粒，表明血清学应答中存在显著的交叉反应性。4份阳性样品对XMRV具有特异性。无论是通过PCR还是血清学方法，在检测的样品中均未观察到XMRV感染与CFS之间的关联。在多份血清样本中观察到的非特异性中和表明，这些反应不太可能由XMRV引起，并强调了基于血清学检测高估XMRV频率的危险。尽管如此，我们认为，在至少4份人血清样本中检测到不抑制VSV-G假型MLV的中和活性表明，一般人群中可能发生XMRV感染，尽管目前结局不确定。
Detection of a retrovirus, xenotropic murine leukaemia virus-related virus (XMRV), has recently been reported in 67% of patients with chronic fatigue syndrome. We have studied a total of 170 samples from chronic fatigue syndrome patients from two UK cohorts and 395 controls for evidence of XMRV infection by looking either for the presence of viral nucleic acids using quantitative PCR (limit of detection <16 viral copies) or for the presence of serological responses using a virus neutralisation assay. We have not identified XMRV DNA in any samples by PCR (0/299). Some serum samples showed XMRV neutralising activity (26/565) but only one of these positive sera came from a CFS patient. Most of the positive sera were also able to neutralise MLV particles pseudotyped with envelope proteins from other viruses, including vesicular stomatitis virus, indicating significant cross-reactivity in serological responses. Four positive samples were specific for XMRV. No association between XMRV infection and CFS was observed in the samples tested, either by PCR or serological methodologies. The non-specific neutralisation observed in multiple serum samples suggests that it is unlikely that these responses were elicited by XMRV and highlights the danger of over-estimating XMRV frequency based on serological assays. In spite of this, we believe that the detection of neutralising activity that did not inhibit VSV-G pseudotyped MLV in at least four human serum samples indicates that XMRV infection may occur in the general population, although with currently uncertain outcomes.
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影响因子：
4.5
作者：
Jern P;Stoye JP;Coffin JM
通讯作者：
Coffin JM
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作者：
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通讯作者：
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3.3
作者：
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通讯作者：
Bannert N