Correlation of collagen fibril properties and inner limiting membrane thickness with vitreoretinal adhesion in human eyes.
Correlation of collagen fibril properties and inner limiting membrane thickness with vitreoretinal adhesion in human eyes.
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人眼中胶原纤维特性和内界膜厚度与玻璃体视网膜粘附的相关性。
DOI：
10.1016/j.exer.2022.109189
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发表时间：
2022-10
影响因子：
3.4
通讯作者：
Coats, B.
中科院分区：
文献类型：
--
作者：
Creveling, C. J.;Alsanea, Y.;Coats, B.
关键词：
玻璃体视网膜粘附强度被认为在各种视网膜疾病中起机械作用;然而，胶原纤维性质和内界膜（ILM）厚度尚未与粘附强度定量相关。在这项工作中，我们量化了胶原纤维密度，角度，长度和内界膜厚度与玻璃体视网膜粘附强度之间的关系，以促进我们对玻璃体视网膜粘附的结构-功能关系的理解。在机械剥离测试之后，提取来自42-89岁供体的赤道和后极的人视网膜切片并进行处理用于透射电子显微镜。将玻璃体视网膜界面处的胶原原纤维分段，并对原纤维密度、角度、长度和ILM厚度进行定量。形态学测量与玻璃体视网膜粘连在同一位置测量。我们发现，在所有年龄组中，赤道部的胶原纤维密度是后极部的1.6倍（p = 0.0305）。在赤道和后极，稳态剥离力与密度增加呈轻微正相关，但仅在赤道具有统计学显著性（p < 0.05）。胶原纤维的角度和长度没有显着变化，年龄或地区。后极部的ILM厚度是赤道部的3.8倍（p < 0.0001）。年龄≤ 60岁的患眼的ILM厚度是年龄> 60岁的患眼的1.8倍（p = 0.0136）。最大剥离力与赤道处ILM厚度的增加显著相关（p = 0.015）。在后极也观察到类似的趋势，但不显著。这些数据表明，胶原有助于在玻璃体视网膜界面处的粘附，但ILM的结构对玻璃体和视网膜之间的分离的开始更有影响。
Vitreoretinal adhesive strength is thought to play a mechanical role in various retinal diseases; however, collagen fibril properties and inner limiting membrane (ILM) thickness have not been quantitatively correlated to adhesive strength. In this work, we quantified the relationship between collagen fibril density, angle, length, and inner limiting membrane thickness with vitreoretinal adhesive strength to advance our understanding of structure-function relationships in vitreoretinal adhesion. Following mechanical peel tests, human retinal sections from the equator and posterior pole of donors 42–89 years of age were extracted and processed for transmission electron microscopy. Collagen fibrils at the vitreoretinal interface were segmented and fibril density, angle, length, and ILM thickness quantified. Morphological measurements were correlated with vitreoretinal adhesion measured in the same location. We found that collagen fibril density was 1.6 times greater in the equator compared to the posterior pole across all ages (p = 0.0305). Steady-state peel force showed a slight positive correlation with increasing density in both the equator and posterior pole, but was only statistically significant in the equator (p < 0.05). Collagen fibril angle and length did not significantly vary with age or region. ILM thickness was 3.8 times thicker in the posterior pole compared to the equator (p < 0.0001). ILM thickness was 1.8 times greater in eyes ≤ 60 years of age compared to eyes > 60 of age (p = 0.0136). Maximum peel force was significantly correlated with increasing ILM thickness in the equator (p = 0.015). A similar trend was seen in the posterior pole, but this was not significant. These data suggest that collagen contributes to adhesion at the vitreoretinal interface, but the structure of the ILM is more influential on the initiation of separation between the vitreous and retina.
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影响因子：
6.9
作者：
Candiello, Joseph;Cole, Gregory J.;Halfter, Willi
通讯作者：
Halfter, Willi
影响因子：
3.4
作者：
BALAZS, EA;TOTH, LZJ;MITCHELL, AP
通讯作者：
MITCHELL, AP
DOI：
10.1007/bf00414796
发表时间：
1975-01-01
期刊：
ALBRECHT VON GRAEFES ARCHIV FUR KLINISCHE UND EXPERIMENTELLE OPHTHALMOLOGIE
影响因子：
--
作者：
FOOS, RY
通讯作者：
FOOS, RY
DOI：
10.1007/bf00414375
发表时间：
1969-01-01
期刊：
Albrecht von Graefes Archiv fur klinische und experimentelle Ophthalmologie. Albrecht von Graefe's archive for clinical and experimental ophthalmology
影响因子：
--
作者：
Gloor, B P
通讯作者：
Gloor, B P
影响因子：
4.4
作者：
Los, LI;van der Worp, RJ;Hooymans, JMM
通讯作者：
Hooymans, JMM