Microfabrication of complex porous tissue engineering scaffolds using 3D projection stereolithography.
Microfabrication of complex porous tissue engineering scaffolds using 3D projection stereolithography.
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DOI：
10.1016/j.biomaterials.2012.01.048
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发表时间：
2012-05
期刊：
影响因子：
14
通讯作者：
Khademhosseini, Ali
中科院分区：
文献类型：
--
作者：
Gauvin, Robert;Chen, Ying-Chieh;Lee, Jin Woo;Soman, Pranav;Zorlutuna, Pinar;Nichol, Jason W.;Bae, Hojae;Chen, Shaochen;Khademhosseini, Ali
关键词：
组织工程的成功将依赖于产生复杂的、细胞接种的三维（3D）结构的能力。因此，可用于精确设计支架材料的结构和形貌的方法将代表功能性组织工程的关键方面。基于自上而下和过程驱动的方法的用于3D支架制造的先前方法由于缺乏对支架结构、孔隙率和细胞相互作用的控制而通常不足以产生复杂的结构。所提出的投影立体光刻（PSL）平台可用于设计复杂的三维组织支架，这些支架可基于计算机辅助设计（CAD）进行工程设计以模仿组织的微结构。PSL系统的开发，编程和优化，以制造3D支架使用明胶甲基丙烯酸酯（GelMA）。结构和预聚物浓度的变化使得能够定制支架的机械性能。利用动态细胞接种方法来提高支架在其整个厚度上的覆盖率。结果表明，孔的互连性允许均匀的人脐静脉内皮细胞（HUVEC）在支架中的分布和增殖，导致在培养期结束时的高细胞密度和汇合。此外，免疫组织化学结果表明，细胞接种在支架上保持其内皮表型，证明了微制造的GelMA支架的生物功能。
The success of tissue engineering will rely on the ability to generate complex, cell seeded three-dimensional (3D) structures. Therefore, methods that can be used to precisely engineer the architecture and topography of scaffolding materials will represent a critical aspect of functional tissue engineering. Previous approaches for 3D scaffold fabrication based on top-down and process driven methods are often not adequate to produce complex structures due to the lack of control on scaffold architecture, porosity, and cellular interactions. The proposed projection stereolithography (PSL) platform can be used to design intricate 3D tissue scaffolds that can be engineered to mimic the microarchitecture of tissues, based on computer aided design (CAD). The PSL system was developed, programmed and optimized to fabricate 3D scaffolds using gelatin methacrylate (GelMA). Variation of the structure and prepolymer concentration enabled tailoring the mechanical properties of the scaffolds. A dynamic cell seeding method was utilized to improve the coverage of the scaffold throughout its thickness. The results demonstrated that the interconnectivity of pores allowed for uniform human umbilical vein endothelial cells (HUVECs) distribution and proliferation in the scaffolds, leading to high cell density and confluency at the end of the culture period. Moreover, immunohistochemistry results showed that cells seeded on the scaffold maintained their endothelial phenotype, demonstrating the biological functionality of the microfabricated GelMA scaffolds.
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影响因子：
14
作者：
Aubin H;Nichol JW;Hutson CB;Bae H;Sieminski AL;Cropek DM;Akhyari P;Khademhosseini A
通讯作者：
Khademhosseini A
影响因子：
2.8
作者：
Han, Li-Hsin;Suri, Shalu;Chen, Shaochen
通讯作者：
Chen, Shaochen
影响因子：
19
作者：
Fozdar, David Y.;Soman, Pranav;Lee, Jin Woo;Han, Li-Hsin;Chen, Shaochen
通讯作者：
Chen, Shaochen
影响因子：
4.9
作者：
Holy, CE;Fialkov, JA;Shoichet, MS
通讯作者：
Shoichet, MS
影响因子：
56.9
作者：
Cukierman, E;Pankov, R;Yamada, KM
通讯作者：
Yamada, KM