Differential effects of plant-based flours on metabolic homeostasis and the gut microbiota in high-fat fed rats.
Differential effects of plant-based flours on metabolic homeostasis and the gut microbiota in high-fat fed rats.
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植物性面粉对高脂喂养大鼠代谢稳态和肠道菌群的差异作用。
DOI：
10.1186/s12986-023-00767-8
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发表时间：
2023-10-19
影响因子：
4.5
通讯作者：
中科院分区：
文献类型：
--
作者：
肠道微生物组是肥胖发展的重要因素，饮食是肠道微生物组的最大调节剂，这突出了更好地了解特定饮食如何改变肠道微生物群以影响代谢疾病的必要性。增加膳食纤维摄入量改变肠道微生物组，改善能量和葡萄糖稳态。膳食纤维存在于各种纤维成分不同的植物面粉中。然而，特定植物基面粉改善能量稳态的比较功效及其发生的机制尚未得到很好的表征。在实验1中，肥胖大鼠被喂食高脂肪饮食（HFD），并补充了四种不同的植物性面粉，持续12周。将大麦粉（BF）、燕麦麸（OB）、小麦麸（WB）和Hi-玉米直链淀粉（HMA）以5%或10%的总纤维含量掺入HFD中，并与HFD对照进行比较。对于实验2，将瘦的、普通饲料喂养的大鼠转换为补充有10%WB或BF的HFD，以确定面粉补充的预防功效。在实验1中，10%BF和10%WB降低体重和肥胖增加，并增加盲肠丁酸。WB和BF处理的大鼠的肠道微生物群分析显示，SCFA产生细菌的相对丰度增加。10%WB和BF也有效预防HFD诱导的肥胖; 10%WB和BF降低体重和肥胖，改善葡萄糖耐量，并降低肝脏和脂肪组织中的炎症标志物和脂肪生成酶表达。这些作用伴随着肠道微生物群的改变，包括与HFD大鼠相比，10%WB和BF大鼠中乳酸杆菌和毛螺菌UCG 001的相对丰度增加，沿着门静脉牛磺脱氧胆酸（TDCA）增加。治疗性和预防性补充10%，而不是5%，WB或BF改善代谢稳态，这可能是由于肠道微生物群诱导的改变。具体而言，这些影响被认为是由于肠丁酸盐和循环TDCA浓度增加。在线版本包含补充材料，可通过10.1186/s12986-023-00767-8获取。
The gut microbiome is a salient contributor to the development of obesity, and diet is the greatest modifier of the gut microbiome, which highlights the need to better understand how specific diets alter the gut microbiota to impact metabolic disease. Increased dietary fiber intake shifts the gut microbiome and improves energy and glucose homeostasis. Dietary fibers are found in various plant-based flours which vary in fiber composition. However, the comparative efficacy of specific plant-based flours to improve energy homeostasis and the mechanism by which this occurs is not well characterized. In experiment 1, obese rats were fed a high fat diet (HFD) supplemented with four different plant-based flours for 12 weeks. Barley flour (BF), oat bran (OB), wheat bran (WB), and Hi-maize amylose (HMA) were incorporated into the HFD at 5% or 10% total fiber content and were compared to a HFD control. For experiment 2, lean, chow-fed rats were switched to HFD supplemented with 10% WB or BF to determine the preventative efficacy of flour supplementation. In experiment 1, 10% BF and 10% WB reduced body weight and adiposity gain and increased cecal butyrate. Gut microbiota analysis of WB and BF treated rats revealed increases in relative abundance of SCFA-producing bacteria. 10% WB and BF were also efficacious in preventing HFD-induced obesity; 10% WB and BF decreased body weight and adiposity, improved glucose tolerance, and reduced inflammatory markers and lipogenic enzyme expression in liver and adipose tissue. These effects were accompanied by alterations in the gut microbiota including increased relative abundance of Lactobacillus and LachnospiraceaeUCG001, along with increased portal taurodeoxycholic acid (TDCA) in 10% WB and BF rats compared to HFD rats. Therapeutic and preventative supplementation with 10%, but not 5%, WB or BF improves metabolic homeostasis, which is possibly due to gut microbiome-induced alterations. Specifically, these effects are proposed to be due to increased concentrations of intestinal butyrate and circulating TDCA. The online version contains supplementary material available at 10.1186/s12986-023-00767-8.
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影响因子：
5.2
作者：
Detman A;Laubitz D;Chojnacka A;Wiktorowska-Sowa E;Piotrowski J;Salamon A;Kaźmierczak W;Błaszczyk MK;Barberan A;Chen Y;Łupikasza E;Yang F;Sikora A
通讯作者：
Sikora A
影响因子：
4.7
作者：
Gart E;van Duyvenvoorde W;Toet K;Caspers MPM;Verschuren L;Nielsen MJ;Leeming DJ;Souto Lima E;Menke A;Hanemaaijer R;Keijer J;Salic K;Kleemann R;Morrison MC
通讯作者：
Morrison MC
影响因子：
3.6
作者：
Meyer, Rachel K.;Bime, Megan A.;Duca, Frank A.
通讯作者：
Duca, Frank A.
DOI：
10.1038/ismej.2012.8
发表时间：
2012-08
期刊：
The ISME journal
影响因子：
--
作者：
通讯作者：
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影响因子：
48
作者：
Callahan BJ;McMurdie PJ;Rosen MJ;Han AW;Johnson AJ;Holmes SP
通讯作者：
Holmes SP