R-100 improves pulmonary function and systemic fluid balance in sheep with combined smoke-inhalation injury and Pseudomonas aeruginosa sepsis.
R-100 improves pulmonary function and systemic fluid balance in sheep with combined smoke-inhalation injury and Pseudomonas aeruginosa sepsis.
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DOI：
10.1186/s12967-017-1366-6
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发表时间：
2017-12-28
影响因子：
7.4
通讯作者：
Enkhbaatar P
中科院分区：
文献类型：
--
作者：
Ito H;Malgerud E;Asmussen S;Lopez E;Salzman AL;Enkhbaatar P
感染性休克是世界各地重症监护病房死亡的主要原因。本研究的目的是研究新型药物R-100（一种超氧化物降解催化剂和一氧化氮供体）是否能改善铜绿假单胞菌和烟雾吸入引起的脓毒性休克羊模型的肺功能。选取11只母羊，经手术准备，在深度麻醉和镇痛下，经支气管镜吸入冷却棉烟，气道内滴入活的铜绿假单胞菌（2.5 × 1011 CFU），随机分为治疗组（n = 5）和对照组（n = 6）。治疗组静脉滴注R-100共80 mg/kg，用5%葡萄糖500 mL稀释。对照组给予5%葡萄糖500 mL。所有动物静脉注射乳酸林格氏液以维持红细胞压积在基线±3%的水平。在基线时测量血气和血流动力学，然后在24小时的研究期间每3小时分析一次。结果用平均值±SEM表示。治疗组动物肺气体交换显著改善（24 h PaO2/FiO2比值为246±29，对照组为90±40，P < 0.05）。治疗组肺动脉压降低（24 h: 26±1 vs 30±2 cm mmHg对照组，P < 0.05）。24 h后，治疗组动物的总体液平衡也有所改善（190±45/24 h mL，对照组为595±234/24 h mL， P < 0.05）。用R-100治疗可改善肺部气体交换和血液氧合，防止吸入烟雾和铜绿假单胞菌的绵羊体液失衡。
Septic shock is a major cause of death in intensive care units around the world . The aim of the study was to investigate whether the novel drug R-100 (a superoxide degradation catalyst and nitric oxide donor) improves pulmonary function in a sheep model of septic shock caused by Pseudomonas aeruginosa and smoke inhalation. Eleven female sheep were prepared surgically and randomly assigned to a treatment group (n = 5) or a control group (n = 6) after inhalation of cooled cotton smoke and airway instillation of live P. aeruginosa (2.5 × 1011 CFU) by bronchoscope under deep anesthesia and analgesia. The treatment group received an intravenous infusion of a total of 80 mg/kg of R-100 diluted in 500 mL of 5% dextrose. The control group was given 500 mL of 5% dextrose. All animals received intravenous lactated Ringer’s solution to maintain a hematocrit level at baseline ± 3%. Blood gas and hemodynamics were measured at baseline and then analyzed every 3 h during the 24-h study period. Results are expressed as mean ± SEM. The treated animals showed significant improvement in their pulmonary gas exchange (PaO2/FiO2 ratio at 24 h: 246 ± 29 vs. 90 ± 40 mmHg control, P < 0.05). Pulmonary arterial pressures were reduced in the treated group (24 h: 26 ± 1 vs. 30 ± 2 cm mmHg control, P < 0.05). The treated animals also had an improved total fluid balance after 24 h (190 ± 45/24 h mL vs. 595 ± 234/24 h mL control, P < 0.05). Treatment with R-100 improves pulmonary gas exchange and blood oxygenation, and prevents a fluid imbalance in sheep subjected to smoke inhalation and P. aeruginosa.
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影响因子：
20.1
作者：
PEARCE, ML;YAMASHITA, J;BEAZELL, J
通讯作者：
BEAZELL, J
DOI：
10.1097/shk.0b013e3181e7e9ba
发表时间：
2010-09
期刊：
Shock (Augusta, Ga.)
影响因子：
--
作者：
Szabó C;Módis K
通讯作者：
Módis K
DOI：
10.1165/rcmb.4860
发表时间：
2003-09-01
影响因子：
6.4
作者：
Cox, RA;Burke, AS;Hawkins, HK
通讯作者：
Hawkins, HK
影响因子：
1.4
作者：
Suzuki, S;Togari, H;Haas, KM
通讯作者：
Haas, KM
DOI：
10.1164/ajrccm.159.3.9801126
发表时间：
1999-03-01
影响因子：
24.7
作者：
Cereda, MF;Sparacino, ME;Kolobow, T
通讯作者：
Kolobow, T