Synchronization of Neuronal Activity Promotes Survival of Individual Rat Neocortical Neurons in Early Development
Synchronization of Neuronal Activity Promotes Survival of Individual Rat Neocortical Neurons in Early Development
复制标题
神经元活动同步促进早期发育中大鼠新皮质神经元的存活
DOI：
10.1111/j.1460-9568.1997.tb01449.x
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发表时间：
1997
影响因子：
3.4
通讯作者：
A. D. Lima
中科院分区：
文献类型：
--
作者：
T. Voigt;H. Baier;A. D. Lima
神经活动被认为在大脑皮层的发育过程中起着重要作用。在这项研究中，我们研究了全球活动块或增强的影响和模式化的射击对培养的大鼠新皮层神经元的能力，在体外的第二周的生存，超越了突触发生的开始。通过加入河豚毒素（TTX）和增加培养基中镁浓度来阻断神经元活性，强烈降低了皮质细胞的存活率。通过提高外部钾浓度来增加神经元活性显著改善了皮层神经元的存活。我们假设在发育中的神经元网络中，神经细胞的存活是由涉及细胞内钙浓度变化的突触介导事件调节的。为了进一步研究这个问题，我们通过同时光学记录许多神经元的细胞内钙信号来监测发育中的网络的活动。这些记录表明，在低镁的新皮层神经元表达同步振荡的细胞内钙浓度。在培养的第二周，网络同步多个细胞的细胞内钙变化的能力逐渐出现，突触密度增加和存活神经元数量下降。通过在记录会话后几天检查识别出的细胞的命运，我们发现与其他神经元共同激活的神经细胞比不参与同步事件的细胞有更高的存活机会。这些实验表明，在早期皮层网络的发展过程中，皮层神经元同步放电活动，神经元的生存至少部分依赖于这种模式的神经元活动。
Neural activity is thought to play a significant role during the development of the cerebral cortex. In this study, we examined the effects of global activity block or enhancement and the effects of patterned firing on the ability of cultured rat neocortical neurons to survive during the second week in vitro, beyond the beginning of synaptogenesis. Blockade of neuronal activity by adding tetrodotoxin (TTX) and increasing magnesium concentration in the medium strongly reduced the survival of cortical cells. Increasing neuronal activity by raising the external potassium concentration significantly improved the survival of cortical neurons. We postulated that in a developing neuronal network the survival of nerve cells is regulated by synaptically mediated events that involve changes in the intracellular calcium concentration. To examine this question further, we monitored the activity of the developing network by optically recording the intracellular calcium signals of many neurons simultaneously. These recordings show that in low magnesium neocortical neurons express synchronized oscillation of their intracellular calcium concentration. The ability of a network to synchronize the changes in intracellular calcium of multiple cells appeared gradually during the second week in culture, paralleled by both an increase in the synaptic density and a decline in the number of surviving neurons. By examining the fate of identified cells several days after a recording session, we found that those nerve cells that were co‐activated with other neurons had a significantly higher chance to survive than cells that did not participate in synchronized events. These experiments demonstrate that during early cortical network development cortical neurons show synchronized firing activity and that the survival of neurons is at least partially dependent on this pattern of neuronal activity.
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影响因子：
56.9
作者：
R. Yuste;A. Peinado;L. C. Katz
通讯作者：
R. Yuste;A. Peinado;L. C. Katz
DOI：
10.1016/s0021-9258(18)83165-9
发表时间：
1989-05
期刊：
The Journal of biological chemistry
影响因子：
--
作者：
A. Minta;J. Kao;R. Tsien
通讯作者：
A. Minta;J. Kao;R. Tsien
影响因子：
56.9
作者：
RAKIC, P;BOURGEOIS, JP;GOLDMANRAKIC, PS
通讯作者：
GOLDMANRAKIC, PS
DOI：
10.1016/0165-3806(89)90124-7
发表时间：
1989-11
期刊：
Brain research. Developmental brain research
影响因子：
--
作者：
Nada Zecevic;Jean-Pierre Bourgeois;P. Rakic
通讯作者：
Nada Zecevic;Jean-Pierre Bourgeois;P. Rakic
影响因子：
56.9
作者：
MEISTER, M;WONG, ROL;SHATZ, CJ
通讯作者：
SHATZ, CJ