Jaceosidin induces apoptosis in human ovary cancer cells through mitochondrial pathway.
Jaceosidin induces apoptosis in human ovary cancer cells through mitochondrial pathway.
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Jaceosidin通过线粒体途径诱导人卵巢癌细胞凋亡。
DOI：
10.1155/2008/394802
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发表时间：
2008
影响因子：
--
通讯作者：
Xie, Xing
中科院分区：
文献类型：
--
作者：
Lv, Wen;Sheng, Xia;Chen, Ting;Xu, Qiang;Xie, Xing
本文研究了从毛蒿中提取的4′，5，7-三羟基-3 ′，6-二甲氧基黄酮（Jaceosidin）对几种人癌细胞系的抑制作用。Jaceosidin以浓度依赖性方式显著降低CAOV-3、SKOV-3、HeLa和PC 3细胞的增殖。Jaceosidin对CAOV-3细胞也有时间依赖性的抑制作用。流式细胞仪分析发现，Jaceosidin处理导致CAOV-3细胞凋亡增加。用Jaceosidin处理的细胞表现出降低的线粒体膜电位。Jaceosidin还增加了切割的caspase-9的水平，并诱导caspase-3和聚（ADP-核糖）聚合酶（PARP）的切割，而caspase-3抑制剂Z-DEVD-FMK显著逆转Jaceosidin在CAOV-3细胞中的促凋亡作用。此外，Jaceosidin提高细胞质中细胞色素c的水平。这些发现表明，Jaceosidin的抗癌作用可能是通过诱导细胞凋亡，涉及细胞色素c从线粒体释放到胞质溶胶。
We examined the antiproliferation effect of Jaceosidin (4′, 5, 7-trihydroxy-3′, 6-dimethoxyflavone) isolated from the herb of Artemisia vestita Wall on several human cancer cell lines. Jaceosidin significantly reduced the proliferation of CAOV-3, SKOV-3, HeLa, and PC3 cells in a concentration-dependent manner. A time-dependent inhibition was also observed in CAOV-3 cells by Jaceosidin. By flow cytometric analysis, we found that Jaceosidin treatment resulted in an increased apoptosis in CAOV-3 cells. The cells treated with Jaceosidin exhibited a decreased mitochondrial membrane potential. Jaceosidin also increased the level of cleaved caspase-9 and induced the cleavage of caspase-3 and poly (ADP-ribose) polymerase (PARP), while caspase-3 inhibitor Z-DEVD-FMK significantly reversed the proapoptotic effect of Jaceosidin in CAOV-3 cells. Moreover, Jaceosidin elevated the level of cytochrome c in cytosol. These findings suggest that the anticancer effect of Jaceosidin may be contributed by an induction of apoptosis involving cytochrome c release from mitochondria to cytosol.
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DOI：
10.1196/annals.1397.052
发表时间：
2007-01-01
期刊：
SIGNAL TRANSDUCTION PATHWAYS, PT C
影响因子：
--
作者：
Kim, Min-Jung;Kim, Do-Hee;Surh, Young-Joon
通讯作者：
Surh, Young-Joon
影响因子：
5.4
作者：
Lee, HG;Yu, KA;Yoon, DY
通讯作者：
Yoon, DY
影响因子：
6.4
作者：
Seo, HJ;Park, KK;Surh, YJ
通讯作者：
Surh, YJ
影响因子：
3.3
作者：
Schinella, GR;Giner, RM;Máñez, S
通讯作者：
Máñez, S
影响因子：
4.2
作者：
Belen Granado-Serrano, Ana;Angeles Martin, Maria;Ramos, Sonia
通讯作者：
Ramos, Sonia