Host perception and signal transduction studies in wild-type Blumeria graminis f. sp. hordei and a quinoxyfen-resistant mutant implicate quinoxyfen in the inhibition of serine esterase activity.
Host perception and signal transduction studies in wild-type Blumeria graminis f. sp. hordei and a quinoxyfen-resistant mutant implicate quinoxyfen in the inhibition of serine esterase activity.
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野生型 Blumeria graminis f 的宿主感知和信号转导研究。
DOI：
10.1002/ps.1538
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发表时间：
2008
影响因子：
4.1
通讯作者：
Lee S
中科院分区：
文献类型：
--
作者：
Lee S
背景：喹氧芬是一种高效杀菌剂，迄今为止被认为是通过干扰早期胚分化过程中的信号转导来控制白粉病。本文的目的是通过比较野生型小麦白粉病菌（Blumeria graminisf）对宿主来源信号和信号传递的感知来了解喹氧芬的作用模式。结果：QR/2B 11比喹氧芬敏感的WT/IM 82在类宿主表面和人工表面上更杂乱地萌发。宿主角质层剥夺在WT/IM 82中钩状附着胞芽管（hAGTs）的形成中的关键作用以及在防霉剂存在下胚分化的急剧下降被证明。QR/2B 11菌株显示hAGT的形成依赖于宿主角质层样特征，但在存在或不存在喹氧芬的情况下，胚分化之间没有显着差异。PKC抑制剂Ro 318220诱导的形态学变化与喹氧芬处理的芽中观察到的相似。在QR/2B 11和WT/IM 82菌株中，喹氧芬同等地上调PKC 1转录物的积累，但QR/2B 11分生孢子中BghcutinaseCUT 1转录物的丰度是WT/IM 82分生孢子中的8倍。喹氧芬抑制WT/IM 82的丝氨酸酯酶活性，但不抑制QR/2B 11的丝氨酸酯酶活性。结论：这些数据表明，喹氧芬干扰了完整胚分化所需的宿主来源信号的感知，而QR/2B 11绕过了对这些信号的需求。此外，喹氧芬似乎靶向丝氨酸酯酶活性，在信号转导中具有下游扰动;这代表了喹氧芬抗性和敏感分离株之间的第一个可证明的生化差异。版权所有© 2008化学工业协会
BACKGROUND:Quinoxyfen is a potent and effective fungicide, hitherto considered to control powdery mildew disease by perturbing signal transduction during early germling differentiation. The aim of this paper is to understand the mode of action of quinoxyfen by comparing the perception of host‐derived signals and signal relay in a wild‐typeBlumeria graminisf. sp.hordeiEM Marchal (Bgh)(WT/IM82) and a quinoxyfen‐resistant field isolate (QR/2B11).RESULTS:QR/2B11 germinates more promiscuously on host‐like and artificial surfaces than the quinoxyfen‐sensitive WT/IM82. The pivotal role of host cuticle deprivation in the formation of hooked appressorial germ tubes (hAGTs) in WT/IM82 and a dramatic drop in germling differentiation in the presence of the mildewicide are demonstrated. QR/2B11 strain shows a dependence on host cuticle‐like features for hAGT formation but no significant difference between germling differentiation in the presence or absence of quinoxyfen. PKC‐inhibitor Ro 318220 induces morphological changes similar to those seen in quinoxyfen‐treated germlings.PKC1transcript accumulation is equivalently upregulated by quinoxyfen in QR/2B11 and WT/IM82 strains, butBghcutinaseCUT1transcript is 8 times more abundant in QR/2B11 conidia than in WT/IM82 conidia. Quinoxyfen inhibits serine esterase activity in WT/IM82, but not in QR/2B11.CONCLUSION:Collectively, these data suggest that quinoxyfen interferes with the perception of host‐derived signals required for full germling differentiation, and that QR/2B11 bypasses the need for such signals. Moreover, quinoxyfen appears to target serine esterase activity, with a downstream perturbation in signal transduction; this represents the first demonstrable biochemical difference between the quinoxyfen‐resistant and ‐sensitive isolates. Copyright © 2008 Society of Chemical Industry
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