Fear conditioning fragments REM sleep in stress-sensitive Wistar-Kyoto, but not Wistar, rats.
Fear conditioning fragments REM sleep in stress-sensitive Wistar-Kyoto, but not Wistar, rats.
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DOI：
10.1016/j.pnpbp.2010.08.023
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发表时间：
2011-01-15
影响因子：
5.6
通讯作者：
Morrison, Adrian R.
中科院分区：
文献类型：
--
作者：
DaSilva, Jamie K.;Lei, Yanlin;Madan, Vibha;Mann, Graziella L.;Ross, Richard J.;Tejani-Butt, Shanaz;Morrison, Adrian R.
巴甫洛夫条件反射常用于研究恐惧学习的机制。由于Wistar-Kyoto（WKY）大鼠品系对压力特别敏感，因此我们研究了WKY大鼠与Wistar（WIS）大鼠相比，心理压力对睡眠的影响。雄性WKY和WIS大鼠要么恐惧条件音线索或单独接受电脚电击。在恐惧调节程序中，动物暴露于10个音调（800 Hz，90 dB，5秒），每个音调与足部电击（1.0 mA，0.5秒）共同终止，间隔30秒。在电击应激过程中，动物以30秒的间隔接受10英尺的电击，没有音调。所有受试者在24小时（第1天）和两周后（第14天）再次接受仅色调测试。通过将快速眼动睡眠（REMS）发作分为单次（REMS发作间隔> 3 min）和连续（间隔≤ 3 min）发作来研究REMS的连续性。在恐惧条件化组中，两种品系的冻结从基线增加，但仅WKY大鼠在第14天保持增加。在恐惧条件化WKY大鼠中，第1天总REMS量增加，第1天和第14天连续REMS量增加，第14天单次REMS量减少。变化是由于连续和单次REMS发作次数的变化。冲击应力对两种品系的REMS微结构均无显著影响。在恐惧条件WKY大鼠中向顺序REMS的转变可能代表REMS片段化，并可能为研究创伤后应激障碍中睡眠障碍的神经生物学机制提供模型。
Pavlovian conditioning is commonly used to investigate the mechanisms of fear learning. Because the Wistar-Kyoto (WKY) rat strain is particularly stress-sensitive, we investigated the effects of a psychological stressor on sleep in WKY compared to Wistar (WIS) rats. Male WKY and WIS rats were either fear-conditioned to tone cues or received electric foot shocks alone. In the fear-conditioning procedure, animals were exposed to 10 tones (800 Hz, 90 dB, 5 sec), each co-terminating with a foot shock (1.0 mA, 0.5 sec), at 30-sec intervals. In the shock stress procedure, animals received 10 foot shocks at 30-sec intervals, without tones. All subjects underwent a tone-only test both 24 hrs (Day 1) and again two weeks (Day 14) later. Rapid eye movement sleep (REMS) continuity was investigated by partitioning REMS episodes into single (inter-REMS episode interval > 3 min) and sequential (interval ≤ 3 min) episodes. In the fear-conditioned group, freezing increased from baseline in both strains, but the increase was maintained on Day 14 in WKY rats only. In fear-conditioned WKY rats, total REMS amount increased on Day 1, sequential REMS amount increased on Day 1 and Day 14, and single REMS amount decreased on Day 14. Alterations were due to changes in the number of sequential and single REMS episodes. Shock stress had no significant effect on REMS microarchitecture in either strain. The shift toward sequential REMS in fear-conditioned WKY rats may represent REMS fragmentation, and may provide a model for investigating the neurobiological mechanisms of sleep disturbances reported in posttraumatic stress disorder.
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影响因子：
17.7
作者：
Mellman, TA;Bustamante, V;Nolan, B
通讯作者：
Nolan, B
影响因子：
5.3
作者：
Datta, S
通讯作者：
Datta, S
影响因子：
3.4
作者：
Mavanji, V;Datta, S
通讯作者：
Datta, S
影响因子：
2.9
作者：
Paré, AMT;Paré, WP;Kluczynski, J
通讯作者：
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影响因子：
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作者：
Breslau, N;Roth, T;Roehrs, T
通讯作者：
Roehrs, T