Atomic force microscopic studies on the structure of bovine femoral cortical bone at the collagen fibril-mineral level
Atomic force microscopic studies on the structure of bovine femoral cortical bone at the collagen fibril-mineral level
复制标题
胶原纤维-矿物质水平上牛股骨皮质骨结构的原子力显微镜研究
DOI：
10.1023/a:1014079421895
复制
发表时间：
2002
期刊：
影响因子：
--
通讯作者：
K. Hikichi
中科院分区：
文献类型：
--
作者：
N. Sasaki;Ayano Tagami;T. Goto;M. Taniguchi;M. Nakata;K. Hikichi
用原子力显微镜观察骨皮质在胶原-矿物水平上的结构。用胶原酶和乙二胺四乙酸（EDTA）处理的标本表面进行检查。在完整标本中观察到的斑点样物体在胶原酶处理后变得清晰；斑点的大小减小，表明每个斑点都被胶原酶处理破碎。在对一个完整的标本进行EDTA处理后，出现了线状物体的图像；线的一部分是由一系列的斑点组成的，而线的其他部分沿着它的长轴有一个周期性的图案。这个周期与Hodge-Petruska模型的胶原蛋白d周期几乎相等，表明丝状物是胶原原纤维，而斑点状物与骨中的矿物相有关。由此得出结论，矿物质沉积在胶原原纤维上和沿胶原原纤维沉积。提出了一种用于描述矿物与胶原纤维空间关系的装饰型胶原纤维模型。根据我们的模型，胶原纤维内部的矿物质大约是纤维外矿物质的四分之一。
The structure of cortical bone at the collagen-mineral level was investigated by means of atomic force microscopy. Surfaces of the specimens treated with collagenase and ethylenediaminetetraacetic acid (EDTA) were examined. Images of blob-like objects observed in intact specimen became clearly outlined after collagenase treatment; the sizes of the blob decreased, suggesting that each blob had been fragmented by the collagenase treatment. Following EDTA treatment of an intact specimen, an image of thread-like objects appeared; the thread was partly constructed by trains of blobs and the other parts of the threads had a periodic pattern along its longer axis. The period was almost equal to the collagen D-period of the Hodge–Petruska model, indicating that the threads are collagen fibrils and that the blobs are related to the mineral phase in bone. It was concluded that minerals were deposited on and along collagen fibrils. A decorated collagen fibril model for the spatial relationship between mineral and collagen fibril was proposed. According to our model, the mineral inside the collagen fibril is about one forth of the extrafibrillar mineral.
登录
查看更多内容
影响因子：
5.6
作者：
BONAR, LC;LEES, S;MOOK, HA
通讯作者：
MOOK, HA
影响因子：
2.9
作者：
Vivi Ziv;S. Weiner
通讯作者：
Vivi Ziv;S. Weiner
影响因子：
3.4
作者：
N. Tao;S. Lindsay;S. Lees
通讯作者：
N. Tao;S. Lindsay;S. Lees
影响因子：
5.6
作者：
LEE, DD;GLIMCHER, MJ
通讯作者：
GLIMCHER, MJ
影响因子：
2.4
作者：
Pidaparti, RMV;Chandran, A;Turner, CH
通讯作者：
Turner, CH