First Asp-2078-Gly Mutation Conferring Resistance to Different ACCase Inhibitors in a Polypogon fugax Population from China.
First Asp-2078-Gly Mutation Conferring Resistance to Different ACCase Inhibitors in a Polypogon fugax Population from China.
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DOI：
10.3390/ijms24010528
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发表时间：
2022-12-28
影响因子：
5.6
通讯作者：
中科院分区：
文献类型：
--
作者：
小亚细亚早熟禾（Polypogon fugax）是我国常见的一种杂草。在油菜田中，常被乙酰辅酶A羧化酶（ACCase）抑制剂控制的P. fugax。在此，我们确认了一个P. fugax种群（R）对测试的所有ACCase抑制剂均表现出耐药性，耐药性指数范围为5.4-18.4。我们进一步研究了该R种群的抗性机制。通过比较敏感群体（S）的ACCase基因序列，分子分析显示在R群体中存在氨基酸突变（Asp-2078-Gly）。此外，在R和S人口之间的敏感性差异不太可能与除草剂代谢。此外，我们还开发了一种新的衍生切割扩增多态性序列（dCAPS）方法，用于有效检测黑曲霉Asp-2078-Gly突变。我们发现，93.75%的R群体中的植物携带Asp-2078-Gly突变，并且该R群体的所有除草剂抗性表型都与该突变分不开。这是第一份关于在P. fugax中由Asp-2078-Gly靶位点突变赋予的ACCase抑制剂交叉耐药性的报告。研究表明，迫切需要提高杂草管理措施的多样性，以防止在中国广泛进化的除草剂抗性。
Asia minor bluegrass (Polypogon fugax) is a common and problematic weed throughout China. P. fugax that is often controlled by acetyl-CoA carboxylase (ACCase) inhibitors in canola fields. Herein, we confirmed a P. fugax population (R) showing resistance to all ACCase inhibitors tested with resistance indexes ranging from 5.4–18.4. We further investigated the resistance mechanisms of this R population. Molecular analyses revealed that an amino acid mutation (Asp-2078-Gly) was present in the R population by comparing ACCase gene sequences of the sensitive population (S). In addition, differences in susceptibility between the R and S population were unlikely to be related to herbicide metabolism. Furthermore, a new derived cleaved amplified polymorphic sequence (dCAPS) method was developed for detecting the Asp-2078-Gly mutation in P. fugax efficiently. We found that 93.75% of plants in the R population carried the Asp-2078-Gly mutation, and all the herbicide-resistant phenotype of this R population is inseparable from this mutation. This is the first report of cross resistance to ACCase inhibitors conferred by the Asp-2078-Gly target-site mutation in P. fugax. The research suggested the urgent need to improve the diversity of weed management practices to prevent the widespread evolution of herbicide resistance in P. fugax in China.
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