Sequencing of Treponema pallidum subsp. pallidum from isolate UZ1974 using Anti-Treponemal Antibodies Enrichment: First complete whole genome sequence obtained directly from human clinical material.
Sequencing of Treponema pallidum subsp. pallidum from isolate UZ1974 using Anti-Treponemal Antibodies Enrichment: First complete whole genome sequence obtained directly from human clinical material.
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DOI：
10.1371/journal.pone.0202619
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发表时间：
2018
期刊：
影响因子：
3.7
通讯作者：
Šmajs D
中科院分区：
文献类型：
--
作者：
Grillová L;Giacani L;Mikalová L;Strouhal M;Strnadel R;Marra C;Centurion-Lara A;Poveda L;Russo G;Čejková D;Vašků V;Oppelt J;Šmajs D
梅毒螺旋体亚种梅毒是一种每年感染超过500万人的疾病。由于TPA是一种不可培养的细菌，大多数关于TPA的遗传信息来自基因组测序和分子分型研究。本研究提出了临床分离株（UZ1974）的第一个完整的TPA基因组（无测序缺口），该基因组直接从临床材料中获得，无需在家兔中增殖。全基因组测序使用新开发的抗密螺旋体抗体富集技术结合先前报道的Pooled片段基因组测序进行。我们鉴定了UW074B基因组，从以前在兔子中繁殖的样本中分离出来，是UZ1974基因组的近亲，并计算出TPA突变率为2.8 x 10−10每个位点每代。
Treponema pallidum subsp. pallidum (TPA) is the infectious agent of syphilis, a disease that infects more than 5 million people annually. Since TPA is an uncultivable bacterium, most of the information on TPA genetics comes from genome sequencing and molecular typing studies. This study presents the first complete TPA genome (without sequencing gaps) of clinical isolate (UZ1974), which was obtained directly from clinical material, without multiplication in rabbits. Whole genome sequencing was performed using a newly developed Anti-Treponemal Antibody Enrichment technique combined with previously reported Pooled Segment Genome Sequencing. We identified the UW074B genome, isolated from a sample previously propagated in rabbits, to be the closest relative of the UZ1974 genome and calculated the TPA mutation rate as 2.8 x 10−10 per site per generation.
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DOI：
10.1128/genomea.00333-14
发表时间：
2014-04-17
期刊：
Genome announcements
影响因子：
--
作者：
Giacani L;Iverson-Cabral SL;King JC;Molini BJ;Lukehart SA;Centurion-Lara A
通讯作者：
Centurion-Lara A
影响因子：
4.2
作者：
Matejkova, Petra;Strouhal, Michal;Smajs, David;Norris, Steven J.;Palzkill, Timothy;Petrosino, Joseph F.;Sodergren, Erica;Norton, Jason E.;Singh, Jaz;Richmond, Todd A.;Molla, Michael N.;Albert, Thomas J.;Weinstock, George M.
通讯作者：
Weinstock, George M.
影响因子：
3.2
作者：
Giacani, Lorenzo;Jeffrey, Brendan M.;Rockey, Daniel D.
通讯作者：
Rockey, Daniel D.
DOI：
10.1086/656533
发表时间：
2010-11-01
期刊：
The Journal of infectious diseases
影响因子：
--
作者：
Marra C;Sahi S;Tantalo L;Godornes C;Reid T;Behets F;Rompalo A;Klausner JD;Yin Y;Mulcahy F;Golden MR;Centurion-Lara A;Lukehart SA
通讯作者：
Lukehart SA
三个treponema pallidum ssp的全基因组序列。 Pertenue菌株：小于基因组序列的0.2％的YAW和梅毒treponemes差异。
影响因子：
3.8
作者：
Cejková D;Zobaníková M;Chen L;Pospíšilová P;Strouhal M;Qin X;Mikalová L;Norris SJ;Muzny DM;Gibbs RA;Fulton LL;Sodergren E;Weinstock GM;Smajs D
通讯作者：
Smajs D