Urchin-like gold nanoparticle-based immunochromatographic strip test for rapid detection of fumonisin B1 in grains
Urchin-like gold nanoparticle-based immunochromatographic strip test for rapid detection of fumonisin B1 in grains
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基于类海胆金纳米粒子的免疫层析试纸条快速检测谷物中的伏马菌素 B1
DOI：
10.1007/s00216-015-8896-7
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发表时间：
2015-08
影响因子：
4.3
通讯作者：
Su Baowei
中科院分区：
文献类型：
--
作者：
Xu Yang;Li Yanping;Ji Yanwei;Su Baowei
为满足粮食样品中伏马菌素B1（FB 1）快速检测的需要，研制了一种基于海胆样金纳米颗粒（UGNs）的免疫层析试纸条（ICS）。的ICS的灵敏度为5.0 ng/mL，这代表了四倍的灵敏度比传统的条制备使用胶体金作为抗体标记的探针。谷物样品中FB 1的分析表明，从条带测试获得的数据与HPLC和酶联免疫吸附测定（ELISA）获得的数据吻合良好。该定性检测不需要任何专门设备，检测时间小于5 min，适用于粮食样品中FB 1的现场检测。总的来说，据我们所知，这是第一次报告使用UGN作为抗体标记的探针，使用ICS灵敏地检测谷物中的FB 1。
分别使用常规胶体金和海胆样金纳米颗粒制备ICS
AbstractAn immunochromatographic strip (ICS) using urchin-like gold nanoparticles (UGNs) for sensitive detection of fumonisin B1 (FB1) was developed to meet the requirement for rapidly monitoring FB1 in grain samples. The sensitivity of the ICS was 5.0 ng/mL, which represents a fourfold increase in sensitivity over conventional strip preparation using colloidal gold as the antibody-labeled probe. Analysis of FB1 in grain samples showed that data obtained from the strip tests were in a good agreement with those obtained from HPLC and enzyme-linked immunosorbent assays (ELISAs). This qualitative test did not require any specialized equipment, and the detection time was less than 5 min, which is suitable for on-site testing of FB1 in grain samples. Overall, to our knowledge, this is the first report of using a UGN as the antibody-labeled probe for sensitive detection of FB1 in grains using an ICS.
Graphical AbstractPreparation of ICS using conventional colloidal gold and urchin-like gold nanoparticle, respectively
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影响因子：
10.4
作者：
Marasas WF
通讯作者：
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影响因子：
1.5
作者：
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通讯作者：
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DOI：
10.1016/j.biocel.2011.03.003
发表时间：
2011-06-01
影响因子：
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通讯作者：
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DOI：
10.1081/txr-120026916
发表时间：
2003-01
期刊：
Journal of Toxicology: Toxin Reviews
影响因子：
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通讯作者：
W. Shier;H. Abbas;M. Abou‐Karam;F. Badria;P. Resch
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作者：
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