Reduced acute inflammatory responses to microgel conformal coatings.
Reduced acute inflammatory responses to microgel conformal coatings.
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DOI：
10.1016/j.biomaterials.2008.08.015
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发表时间：
2008-12
期刊：
影响因子：
14
通讯作者：
Garcia, Andres J.
中科院分区：
文献类型：
--
作者：
Bridges, Amanda W.;Singh, Neetu;Burns, Kellie L.;Babensee, Julia E.;Lyon, L. Andrew;Garcia, Andres J.
将合成材料植入体内会引发炎症宿主反应，从而限制医疗器械的整合和生物学性能。这种炎症级联反应涉及蛋白质吸附、白细胞募集和激活、细胞因子释放和植入物的纤维包裹。我们提出了一种基于聚（N-异丙基丙烯酰胺）水凝胶微粒（即微凝胶）与聚（乙二醇）二丙烯酸酯交联的薄膜的涂层策略。将这些颗粒接枝到临床相关的聚合物材料上以产生保形涂层，其显著降低体外纤维蛋白原吸附和原代人单核细胞/巨噬细胞粘附和扩散。这些微凝胶涂层还减少了白细胞粘附和促炎细胞因子（TNF-α、IL-1β、MCP-1）的表达，以响应急性植入小鼠腹腔内空间的材料。这些微凝胶涂层可作为保护涂层应用于生物医学植入物，以减弱生物污损、白细胞粘附和活化以及生物医学和生物技术应用的不良宿主反应。
Implantation of synthetic materials into the body elicits inflammatory host responses that limit medical device integration and biological performance. This inflammatory cascade involves protein adsorption, leukocyte recruitment and activation, cytokine release, and fibrous encapsulation of the implant. We present a coating strategy based on thin films of poly(N-isopropylacrylamide) hydrogel microparticles (i.e. microgels) cross-linked with poly(ethylene glycol) diacrylate. These particles were grafted onto a clinically relevant polymeric material to generate conformal coatings that significantly reduced in vitro fibrinogen adsorption and primary human monocytes/macrophage adhesion and spreading. These microgel coatings also reduced leukocyte adhesion and expression of pro-inflammatory cytokines (TNF-α, IL-1β, MCP-1) in response to materials implanted acutely in the murine intraperitoneal space. These microgel coatings can be applied to biomedical implants as a protective coating to attenuate biofouling, leukocyte adhesion and activation, and adverse host responses for biomedical and biotechnological applications.
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