OPTIMIZATION OF A MICROFLUIDIC DEVICE FOR DIFFUSION-BASED EXTRACTION OF DMSO FROM A CELL SUSPENSION.

OPTIMIZATION OF A MICROFLUIDIC DEVICE FOR DIFFUSION-BASED EXTRACTION OF DMSO FROM A CELL SUSPENSION.
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DOI:
10.1016/j.ijheatmasstransfer.2008.04.018
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发表时间:
2008-11
影响因子:
5.2
通讯作者:
Hubel, A.
Hubel, A.
中科院分区:
工程技术2区
文献类型:
--
作者:
Glass, K. K. Fleming;Longmire, E. K.;Hubel, A.

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本研究考虑使用双流微流体装置从冷冻保存的细胞悬浮液中提取二甲亚砜 (DMSO)。 DMSO 从细胞悬浮液流扩散到并行流动的相邻洗涤流中。弗莱明等人的模型。用于确定和讨论需要从体积流速高达 2.5 ml/min 的悬浮液流中去除 95% DMSO 的情况的最佳几何形状和操作条件。分析了佩克莱特数、流速分数和细胞体积分数的影响,并讨论了该分析扩展到其他应用。
This study considers the use of a two-stream microfluidic device for extraction of dimethyl sulphoxide (DMSO) from a cryopreserved cell suspension. The DMSO diffuses from a cell suspension stream into a neighboring wash stream flowing in parallel. The model of Fleming et al. is employed to determine and discuss optimal geometry and operating conditions for a case requiring removal of 95% DMSO from suspension streams with volumetric flow rates up to 2.5 ml/min. The effects of Peclet number, flow rate fraction, and cell volume fraction are analyzed, and expansion of the analysis to other applications is discussed.
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