Pulsed laser activated cell sorting with three dimensional sheathless inertial focusing.
Pulsed laser activated cell sorting with three dimensional sheathless inertial focusing.
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DOI：
10.1002/smll.201302885
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发表时间：
2014-05-14
期刊：
影响因子：
13.3
通讯作者：
Chiou, Pei-Yu
中科院分区：
文献类型：
--
作者：
Chen, Yue;Chung, Aram J.;Wu, Ting-Hsiang;Teitell, Michael A.;Di Carlo, Dino;Chiou, Pei-Yu
我们提出了一种集成了3D无鞘惯性聚焦的脉冲激光激活细胞分选仪（PLACS），能够以每秒10，000个粒子的速度分选，分选纯度>90%，每秒6，000个细胞，分选纯度>80%。它是通过在单层PDMS微流体通道中激发激光诱导空化气泡来实现的，以产生高速液体射流来偏转检测到的荧光样品。利用流体惯性和阶梯微通道引起的二次流，实现了样品的三维聚焦。聚焦后，样品通过膨胀室，膨胀室的功能是通过惯性流中的粒子-粒子相互作用来扩大两个紧密定位的粒子之间的分离距离，并减少切换窗口（时间为16 μsec或距离为32 μm）内的重合事件。该分选机使用比基于鞘的PLACS低10倍的初始浓度细胞样品，以避免在相同通量下来自高体积鞘流的严重稀释效应。
We present a Pulsed Laser Activated Cell Sorter (PLACS) integrated with 3D sheathless inertial focusing that is capable to sort at 10,000 particles sec−1with >90% sort purity and 6,000 cells sec−1with >80% sort purity. It is realized by exciting laser induced cavitation bubbles in a single layer PDMS microfluidic channel to create high speed liquid jets to deflect detected fluorescent samples. Fluid inertia and secondary flows induced by stepped microchannels are used to focus samples in 3 dimensions. After focusing, samples go through an expansion chamber whose function is to enlarge the separation distance between two closely positioned particles by particle-particle interaction in inertial flows and reduce the coincident events within the switching window (16 μsec in time or 32 μm in distance). This sorter uses 10 times lower initial concentration cell samples than that in sheath-based PLACS in order to avoid severe dilution effects from high volume sheath flows at the same throughput.
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影响因子：
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