The Effect of Selenium Supplementation on Glucose Homeostasis and the Expression of Genes Related to Glucose Metabolism.
The Effect of Selenium Supplementation on Glucose Homeostasis and the Expression of Genes Related to Glucose Metabolism.
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DOI：
10.3390/nu8120772
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发表时间：
2016-12-13
期刊：
影响因子：
5.9
通讯作者：
Wasowicz W
中科院分区：
文献类型：
--
作者：
Jablonska E;Reszka E;Gromadzinska J;Wieczorek E;Krol MB;Raimondi S;Socha K;Borawska MH;Wasowicz W
本研究的目的是评估硒补充对人体葡萄糖代谢相关基因表达的影响，以解释硒与糖尿病风险之间不明确的关系。为了进行基因表达分析，我们使用了先前研究中补充硒的76名非糖尿病受试者的cDNA档案样本。补充周期为6周，硒的日剂量为200µg（以酵母硒的形式）。在四个时间点采集血液进行mRNA分离：补充前，补充2周和4周后，以及洗脱期4周后。该分析包括15个基因，这些基因编码了与胰岛素信号传导和葡萄糖代谢有关的选定蛋白质。此外，分别在3个和4个时间点测量HbA1c和空腹血糖。补充硒与HbA1c水平显著降低相关，但与空腹血糖（FPG）水平无关，并与7个基因（INSR、ADIPOR1、LDHA、PDHA、PDHB、MYC和HIF1AN）的显著下调相关。这些结果表明，硒可能在不同的调节水平上影响血糖控制，与胰岛素信号、糖酵解和丙酮酸代谢有关。需要进一步研究这种转录调控的机制及其在直接代谢作用中的潜在意义。
The aim of the study was to evaluate the effect of selenium supplementation on the expression of genes associated with glucose metabolism in humans, in order to explain the unclear relationship between selenium and the risk of diabetes. For gene expression analysis we used archival samples of cDNA from 76 non-diabetic subjects supplemented with selenium in the previous study. The supplementation period was six weeks and the daily dose of selenium was 200 µg (as selenium yeast). Blood for mRNA isolation was collected at four time points: before supplementation, after two and four weeks of supplementation, and after four weeks of washout. The analysis included 15 genes encoding selected proteins involved in insulin signaling and glucose metabolism. In addition, HbA1c and fasting plasma glucose were measured at three and four time points, respectively. Selenium supplementation was associated with a significantly decreased level of HbA1c but not fasting plasma glucose (FPG) and significant down-regulation of seven genes: INSR, ADIPOR1, LDHA, PDHA, PDHB, MYC, and HIF1AN. These results suggest that selenium may affect glycemic control at different levels of regulation, linked to insulin signaling, glycolysis, and pyruvate metabolism. Further research is needed to investigate mechanisms of such transcriptional regulation and its potential implication in direct metabolic effects.
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