Collagenous matrix supported by a 3D-printed scaffold for osteogenic differentiation of dental pulp cells.
Collagenous matrix supported by a 3D-printed scaffold for osteogenic differentiation of dental pulp cells.
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DOI：
10.1016/j.dental.2017.10.001
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发表时间：
2018-03
期刊：
影响因子：
--
通讯作者：
Tayebi L
中科院分区：
文献类型：
--
作者：
Fahimipour F;Dashtimoghadam E;Rasoulianboroujeni M;Yazdimamaghani M;Khoshroo K;Tahriri M;Yadegari A;Gonzalez JA;Vashaee D;Lobner DC;Jafarzadeh Kashi TS;Tayebi L
系统地表征了基于组合添加剂制造技术和冷冻干燥方法制备的复合支架，为牙髓细胞的成骨分化提供了新的平台。支架由嵌入3D打印的β-磷酸三钙(β-TcP)的胶原基组成，作为矿物相。由于3D打印的β-TCP支架和与胶原细胞外基质有关的具有生物活性的3D细胞培养基质，本结构设计旨在实现机械稳定性。研究了β-TcP前驱体配方在不同温度下的流动性能，以优化其制备具有相互连接的孔洞的3D打印支架。用三维激光扫描显微镜、X射线衍射、傅立叶变换红外光谱和抗压强度测试对所开发的结构进行了表征。支架的体外表征表明，与3D打印的β-tCP支架相比，β-tCP/胶原杂化构建的支架在三周内具有更好的DPC增殖和碱性磷酸酶活性。此外，还发现在胶原基质中掺入了磷酸三钙，提高了碱性磷酸酶的活性。结果表明，3D打印的β-TcP/胶原杂化构建物可作为DPC向成骨细胞分化的新平台，用于颅颌面骨再生。
A systematic characterization of hybrid scaffolds fabricated based on combinatorial additive manufacturing technique and freeze-drying method are presented as a new platform for osteoblastic differentiation of dental pulp cells (DPCs). The scaffolds were consisted of a collagenous matrix embedded in a 3D-printed beta-tricalcium phosphate (β-TCP) as the mineral phase. The presented construct design was intended to achieve mechanical robustness owing to 3D-printed β-TCP scaffold, and biologically active 3D cell culture matrix pertaining to the collagen extracellular matrix. The β-TCP precursor formulations were investigated for their flow-ability at various temperatures to be optimized for fabrication of 3D printed scaffolds with interconnected porosity. The developed constructs were characterized by 3D laser scanning microscopy, X-ray diffraction, Fourier transform infrared spectroscopy, and compressive strength testing. The in vitro characterization of scaffolds revealed that the hybrid β-TCP/Collagen constructs offer superior DPCs proliferation and alkaline phosphatase (ALP) activity compared to the 3D-printed β-TCP scaffold over three weeks. Moreover, it was found that the incorporation of TCP into the collagen matrix improves the ALP activity. The obtained results converge to suggest the developed 3D-printed β-TCP/Collagen hybrid constructs as a new platform for osteoblastic differentiation of DPCs for craniomaxillofacial bone regeneration.
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影响因子：
14
作者：
Kim, SS;Park, MS;Kim, BS
通讯作者：
Kim, BS
DOI：
10.1016/j.msec.2016.07.011
发表时间：
2016-12-01
影响因子：
7.9
作者：
Khojasteh, Arash;Fahimipour, Farahnaz;Tayebi, Lobat
通讯作者：
Tayebi, Lobat
DOI：
10.1590/s1516-14392014000400021
发表时间：
2014-08-01
期刊：
Materials Research
影响因子：
--
作者：
Moreira, Ana Paula Duarte;Sader, Márcia Soares;Leão, Maria Helena Miguez Rocha
通讯作者：
Leão, Maria Helena Miguez Rocha
影响因子：
5.6
作者：
FRASER, RDB;MACRAE, TP;SUZUKI, E
通讯作者：
SUZUKI, E
影响因子：
5.6
作者：
MILES, CA;BURJANADZE, TV;BAILEY, AJ
通讯作者：
BAILEY, AJ