The neostriatal mosaic: III. Biochemical and developmental dissociation of patch-matrix mesostriatal systems
The neostriatal mosaic: III. Biochemical and developmental dissociation of patch-matrix mesostriatal systems
复制标题
新纹状体镶嵌：III。
作者：
C. Gerfen;K. Baimbridge;J. Thibault
在先前的论文中（Gerfen等人，1987）中纹状体多巴胺能神经元被细分为背侧和腹侧层，分别投射到纹状体基质和斑隔室。本研究提供的实验证据表明，这些补丁基质中纹状体多巴胺能系统是生化和发育不同。一种28 kDa的钙结合蛋白（CaBP，或calbindin-D28 kDa）在背侧层中纹状体多巴胺能神经元中表达。这些神经元分布在腹侧被盖区、黑质背侧层和红核后区，与投射到纹状体基质的多巴胺能神经元的分布相匹配。不表达CaBP的多巴胺能神经元--那些位于腹侧层的丘脑腹侧部和网状部的多巴胺能神经元--以与多巴胺能投射到纹状体斑块的起源相匹配的模式分布。在发育过程中，多巴胺能传入纹状体补丁隔室是在适当的发展之前，那些矩阵。将神经毒素6-羟基多巴胺（6-OHDA）注射到新生大鼠的纹状体中，导致纹状体斑片中多巴胺能传入神经的选择性和持久性耗竭。这种病变相对不影响后期发展的基质投射.存活的多巴胺能神经元的分布，标记与酪氨酸羟化酶（TH）的免疫反应，匹配的模式，背层神经元先前显示提供输入的矩阵。存活的神经元也表达CaBP免疫反应性，并有树突，传播mediolatively，在飞机的pars延髓。另一方面，这些神经元项目的补丁选择性病变的新生儿6-OHDA纹状体注射，不表达CaBP，并有树突腹侧到网状部。
In the previous paper (Gerfen et al., 1987) mesostriatal dopaminergic neurons were shown to be subdivided into dorsal and ventral tiers that project to the striatal matrix and patch compartments, respectively. The present study provides experimental evidence that these patch- matrix mesostriatal dopaminergic systems are biochemically and developmentally distinct. A 28 kDa calcium-binding protein (CaBP, or calbindin-D28 kDa) is expressed in dorsal tier mesostriatal dopaminergic neurons. The distribution of such neurons, located in the ventral tegmental area, dorsal tier of the substantia nigra pars compacta, and retrorubral area, matches that of dopaminergic neurons that project to the striatal matrix. Dopaminergic neurons that do not express CaBP--those in the ventral tier of the pars compacta and in the pars reticulata--are distributed in a pattern that matches the origin of the dopaminergic projection to the striatal patches. During development, dopaminergic afferents to the striatal patch compartment are in place prior to the development of those to the matrix. Injections of the neurotoxin 6-hydroxydopamine (6-OHDA) into the striatum of newborn rats result in a selective and long-lasting depletion of dopaminergic afferents in the striatal patches. The later- developing matrix projection is relatively spared by such lesions. The distribution of surviving dopaminergic neurons, labeled with tyrosine hydroxylase (TH) immunoreactivity, matches the pattern of dorsal tier neurons previously shown to provide inputs to the matrix. Surviving neurons also express CaBP immunoreactivity and have dendrites that spread mediolaterally, in the plane of the pars compacta. On the other hand, those neurons that project to the patches are selectively lesioned by the neonatal 6-OHDA striatal injections, do not express CaBP, and have dendrites that are directed ventrally into the pars reticulata.
A10 多巴胺神经元：自身受体在确定放电率和对多巴胺激动剂敏感性中的作用。
影响因子：
6.1
作者：
White,FJ;Wang,RY
通讯作者：
Wang,RY
纹状体阿片受体：突触前和突触后定位。
影响因子：
6.1
作者：
Murrin,LC;Coyle,JT;Kuhar,MJ
通讯作者：
Kuhar,MJ
影响因子：
56.9
作者：
WHITE, FJ;WANG, RY
通讯作者：
WANG, RY