Monitoring response to chemotherapy of non-Hodgkin's lymphoma xenografts by T(2)-weighted and diffusion-weighted MRI.
Monitoring response to chemotherapy of non-Hodgkin's lymphoma xenografts by T(2)-weighted and diffusion-weighted MRI.
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DOI：
10.1002/nbm.1261
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发表时间：
2008-11
影响因子：
2.9
通讯作者：
Glickson JD
中科院分区：
文献类型：
--
作者：
Huang MQ;Pickup S;Nelson DS;Qiao H;Xu HN;Li LZ;Zhou R;Delikatny EJ;Poptani H;Glickson JD
一种有效的体内检测非霍奇金淋巴瘤患者早期治疗反应的方法，将使癌症治疗的个性化临床管理和优化治疗方案的设计成为可能。本研究评估了T2WI加权MRI （T2WI）和弥散加权MRI （DWI）在体内检测严重联合免疫缺陷小鼠人类弥漫性大b细胞淋巴瘤异种移植对化疗反应的可行性。每个周期的联合化疗与环磷酰胺，羟多柔比星，Oncovin，强的松，苔藓抑素1 （CHOPB）给予肿瘤携带小鼠每周最多四个周期。在CHOPB开始前和每个周期后分别进行T2WI和DWI检查。为了证实MRI结果，在完成所有MRI研究后，对对照肿瘤和治疗肿瘤进行组织学分析。DWI显示，早在CHOPB开始后1周，CHOPB治疗的肿瘤的平均表观扩散系数就显著增加（P < 0.03）。然而，仅在两个CHOPB周期后，平均T2才出现显著下降（P < 0.03）。基于局部表观扩散系数和T2，两种MRI方法均产生高分辨率（0.1 × 0.1 × 1.0 mm3）的治疗肿瘤区域治疗反应图。只有肿瘤的一个特定区域（5个肿瘤中的3个）约占肿瘤体积的三分之一，表现出与应答相关的ADC升高和T2降低。邻近区域T2升高，ADC无变化。根据MRI标准，其余肿瘤与假药对照组无法区分。MRI检测到的治疗肿瘤的治疗反应伴随着肿瘤细胞密度、增殖和凋亡的变化，这些变化是在纵向研究完成后进行的组织学研究显示的。产生肿瘤局部反应的机制仍有待阐明。
An effective method for in vivo detection of early therapeutic response of patients with non-Hodgkin’s lymphoma would enable personalized clinical management of cancer therapy and facilitate the design of optimal treatment regimens. This study evaluates the feasibility of T2-weighted MRI (T2WI) and diffusion-weighted MRI (DWI) for in vivo detection of response of human diffuse large B-cell lymphoma xenografts in severe combined immunodeficient mice to chemotherapy. Each cycle of combination chemotherapy with cyclophosphamide, hydroxydoxorubicin, Oncovin, prednisone, and bryostatin 1 (CHOPB) was administered to tumor-carrying mice weekly for up to four cycles. T2WI and DWI were performed before the initiation of CHOPB and after each cycle of CHOPB. In order to corroborate the MRI results, histological analyses were carried out on control tumors and treated tumors after completion of all MRI studies. DWI revealed a significant (P < 0.03) increase in the mean apparent diffusion coefficient in CHOPB-treated tumors as early as 1 week after initiation of CHOPB. However, a significant (P < 0.03) decrease in mean T2 was observed only after two cycles of CHOPB. Both MRI methods produced high-resolution (0.1 × 0.1 × 1.0 mm3) maps of regional therapeutic response in the treated tumors based on local apparent diffusion coefficient and T2. Only a specific region of the tumors (in 3 of the 5 tumors) corresponding to about one third of the tumor volume exhibited a response-associate increase in ADC and decrease in T2. An adjacent region exhibited an increase in T2 and no change in ADC. The rest of the tumor was indistinguishable from sham-treated controls by MRI criteria. The therapeutic response of the treated tumors detected by MRI was accompanied by changes in tumor cell density, proliferation and apoptosis revealed by histological studies performed upon completion of the longitudinal study. The mechanism producing the regional response of the tumor remains to be elucidated.
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影响因子：
3.3
作者：
Ababneh, Z;Beloeil, H;Mulkern, RV
通讯作者：
Mulkern, RV
影响因子：
4
作者：
Gauvain, KM;Garbow, JR;Weilbaecher, K
通讯作者：
Weilbaecher, K
影响因子：
4.7
作者：
Issels, Rolf D;Schlemmer, Marcus;Lindner, Lars H
通讯作者：
Lindner, Lars H
DOI：
10.1016/s0360-3016(96)00371-9
发表时间：
1996-10-01
影响因子：
7
作者：
Bhujwalla, ZM;Glickson, JD
通讯作者：
Glickson, JD
DOI：
10.1016/0167-4889(84)90042-9
发表时间：
1984-01-01
期刊：
BIOCHIMICA ET BIOPHYSICA ACTA
影响因子：
--
作者：
EVANOCHKO, WT;SAKAI, TT;GLICKSON, JD
通讯作者：
GLICKSON, JD