Nonconformity of biofilm formation in vivo and in vitro based on Staphylococcus aureus accessory gene regulator status.
Nonconformity of biofilm formation in vivo and in vitro based on Staphylococcus aureus accessory gene regulator status.
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DOI：
10.1038/s41598-022-05382-w
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发表时间：
2022-01-24
影响因子：
4.6
通讯作者：
Daly SM
中科院分区：
文献类型：
--
作者：
Jordan SC;Hall PR;Daly SM
金黄色葡萄球菌是一种机会性致病菌，可引起显著的发病率和死亡率。由于金黄色葡萄球菌的抗生素耐药性仍然是一个严重的问题，开发新的药物疗法来对抗这些感染是至关重要的。群体感应抑制剂（Quorum sensing inhibitors， QSI）通过阻断群体感应信号传导，抑制金黄色葡萄球菌的毒力，促进宿主免疫系统的清除。群体感应信号传导促进了由辅助基因调控子（agr）操纵子控制的毒力基因的上调。虽然qsi在金黄色葡萄球菌皮肤感染的小鼠模型中显示出治疗前景，但它们的进一步发展受到agr抑制促进生物膜形成的建议的阻碍。在这些研究中，我们研究了不同金黄色葡萄球菌菌株和实验条件下agr功能与生物膜生长之间的关系，包括在植入物相关感染的小鼠模型中。我们发现agr缺失仅在狭窄的体外条件下与生物膜的增加有关，关键的是，与生物膜发育增强或体内发病率增加无关。
Staphylococcus aureus is an opportunistic, pathogenic bacteria that causes significant morbidity and mortality. As antibiotic resistance by S. aureus continues to be a serious concern, developing novel drug therapies to combat these infections is vital. Quorum sensing inhibitors (QSI) dampen S. aureus virulence and facilitate clearance by the host immune system by blocking quorum sensing signaling that promotes upregulation of virulence genes controlled by the accessory gene regulator (agr) operon. While QSIs have shown therapeutic promise in mouse models of S. aureus skin infection, their further development has been hampered by the suggestion that agr inhibition promotes biofilm formation. In these studies, we investigated the relationship between agr function and biofilm growth across various S. aureus strains and experimental conditions, including in a mouse model of implant-associated infection. We found that agr deletion was associated with the presence of increased biofilm only under narrow in vitro conditions and, crucially, was not associated with enhanced biofilm development or enhanced morbidity in vivo.
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影响因子：
3.1
作者：
Beenken, KE;Blevins, JS;Smeltzer, MS
通讯作者：
Smeltzer, MS
影响因子：
5.2
作者：
Cheung GYC;Bae JS;Otto M
通讯作者：
Otto M
影响因子：
3.9
作者：
Lade, Harshad;Park, Joon Hyun;Kim, Jae-Seok
通讯作者：
Kim, Jae-Seok
影响因子：
6.7
作者：
del Pozo, J. L.;Patel, R.
通讯作者：
Patel, R.
通过SRNA抑制作用的范围群体传感抑制剂。
影响因子：
4.6
作者：
Jakobsen TH;Warming AN;Vejborg RM;Moscoso JA;Stegger M;Lorenzen F;Rybtke M;Andersen JB;Petersen R;Andersen PS;Nielsen TE;Tolker-Nielsen T;Filloux A;Ingmer H;Givskov M
通讯作者：
Givskov M