Sandwich ELISA-Based Electrochemical Biosensor for Leptin in Control and Diet-Induced Obesity Mouse Model.
Sandwich ELISA-Based Electrochemical Biosensor for Leptin in Control and Diet-Induced Obesity Mouse Model.
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DOI：
10.3390/bios11010007
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发表时间：
2020-12-24
期刊：
影响因子：
--
通讯作者：
Heo YS
中科院分区：
文献类型：
--
作者：
Sung R;Heo YS
瘦素是主要在脂肪组织中产生的肽激素。瘦素被认为是与肥胖和肥胖介导的疾病相关的生物标志物。生物传感器检测血液中的瘦素可能作为动态病理变化的指标发挥关键作用。本文介绍了一种采用丝网印刷金电极（SPGEs）为基底的邻苯二胺（oPD）电化学生物传感器，用于检测饮食诱导肥胖（DIO）小鼠模型的瘦素。在0.1 - 20 ng/mL范围内获得了瘦素浓度的线性校准曲线，检测下限为0.033 ng/mL。采用循环伏安法（CV）和方波伏安法（SWV）两种伏安法，用辣根过氧化物酶（HRP）催化的oPD氧化定量测定瘦素浓度。所提出的夹心酶联免疫吸附测定（ELISA）为基础的电化学生物传感器的小鼠血清中的瘦素显示出较高的稳定性，灵敏度，选择性和有效性相比，商业瘦素ELISA测量。因此，我们相信这种瘦素生物传感器可以成为一种灵敏的分析工具，用于检测临床和即时检测（POCT）中的低水平生物标志物。
Leptin is a peptide hormone produced primarily in adipose tissues. Leptin is considered a biomarker associated with obesity and obesity-mediated diseases. Biosensor detection of leptin in the blood may play a critical role as an indicator of dynamic pathological changes. In this paper, we introduce an electrochemical biosensor that adopts o-Phenylenediamine (oPD) on screen-printed gold electrodes (SPGEs) for detecting the leptin from a mouse model of diet-induced obesity (DIO). A linear calibration curve for the leptin concentration was obtained in the ranges from 0.1 to 20 ng/mL with a lower detection limit of 0.033 ng/mL. The leptin concentration was quantified with HRP (horseradish peroxidase)-catalyzed oxidation of oPD by two voltammetry methods: cyclic voltammetry (CV) and square-wave voltammetry (SWV). The proposed sandwich enzyme-linked immunosorbent assay (ELISA)-based electrochemical biosensor for the leptin in mouse blood serum showed high stability, sensitivity, selectivity, and effectivity compared to the commercial Leptin ELISA measurement. Thus, we believe that this leptin biosensor can be a sensitive analytical tool to detect low-levels of biomarkers in clinics and point-of-care testing (POCT).
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