Dietary S. maltophilia induces supersized lipid droplets by enhancing lipogenesis and ER-LD contacts in C. elegans.
Dietary S. maltophilia induces supersized lipid droplets by enhancing lipogenesis and ER-LD contacts in C. elegans.
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饮食中的嗜麦芽糖链球菌通过增强秀丽隐杆线虫的脂肪生成和 ER-LD 接触来诱导超大脂滴
DOI：
10.1080/19490976.2021.2013762
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发表时间：
2022-01
期刊：
影响因子：
12.2
通讯作者：
Liu P
中科院分区：
文献类型：
--
作者：
Xie K;Liu Y;Li X;Zhang H;Zhang S;Mak HY;Liu P
摘要食源菌和共生菌对后生动物的脂类代谢有重要影响。最近的研究表明，微生物群在动物肥胖和相关的健康疾病中发挥作用，但细菌影响宿主脂质储存的机制尚不清楚。为了减少肠道菌群与宿主之间关系的复杂性，秀丽隐杆线虫（C. elegans）被选为研究种间相互作用的模式生物。在这里，我们证明了喂养C。elegans与条件致病性细菌嗜麦芽寡养单胞菌（Stenotrophomonasmaltophilia，S.嗜麦芽窄食单胞菌）阻碍生长并促进过度的中性脂质储存。基因表达分析表明，膳食S。嗜麦芽窄食单胞菌诱导包括SREBP直系同源物SBP-1和脂肪酸去饱和酶FAT-6和FAT-7的脂肪生成转录应答。活体成像和超微结构分析表明，多余的中性脂质储存在大大扩展的脂滴（LD），作为增强内质网（ER）-LD相互作用的结果。我们还报道了C. elegans赋予对S.嗜麦芽窄食症饮食S。嗜麦芽窄食单胞菌通过增强C.优雅这项工作描绘了一个新的模式，了解微生物调节后生动物的生理。
ABSTRACT Dietary and symbiotic bacteria can exert powerful influence on metazoan lipid metabolism. Recent studies have emerged that microbiota have a role in animal obesity and related health disorders, but the mechanisms by which bacteria influence lipid storage in their host are unknown. To reduce the complexity of the relationship between gut microbiota and the host, Caenorhabditis elegans (C. elegans) has been chosen as a model organism to study interspecies interaction. Here, we demonstrate that feeding C. elegans with an opportunistic pathogenic bacterium Stenotrophomonas maltophilia (S. maltophilia) retards growth and promotes excessive neutral lipid storage. Gene expression analysis reveals that dietary S. maltophilia induces a lipogenic transcriptional response that includes the SREBP ortholog SBP-1, and fatty acid desaturases FAT-6 and FAT-7. Live imaging and ultrastructural analysis suggest that excess neutral lipid is stored in greatly expanded lipid droplets (LDs), as a result of enhanced endoplasmic reticulum (ER)-LD interaction. We also report that loss of function mutations in dpy-9 in C. elegans confers resistance to S. maltophilia. Dietary S. maltophilia induces supersized LDs by enhancing lipogenesis and ER-LD contacts in C. elegans. This work delineates a new model for understanding microbial regulation of metazoan physiology.
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影响因子：
3.7
作者：
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通讯作者：
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3.3
作者：
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DOI：
10.1083/jcb.201502070
发表时间：
2015-11-23
期刊：
The Journal of cell biology
影响因子：
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作者：
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通讯作者：
Carvalho P