Influence of biological sex and exercise on murine cardiac metabolism.
Influence of biological sex and exercise on murine cardiac metabolism.
复制标题
生物学性别和运动对小鼠心脏代谢的影响。
DOI：
10.1016/j.jshs.2022.06.001
复制
发表时间：
2022-07
影响因子：
11.7
通讯作者：
Hill, Bradford G.
中科院分区：
文献类型：
--
作者：
Fulghum, Kyle;Collins, Helen E.;Jones, Steven P.;Hill, Bradford G.
生物性别对心脏代谢物丰度有相当大的影响。女性心脏比男性心脏表现出更多的运动诱导的代谢物变化。心脏代谢物的变化在24小时内恢复到基线水平。运动不影响心脏线粒体呼吸在孤立的细胞器。女性心脏可能比男性心脏具有更大的内源性腺苷二磷酸敏感性。虽然运动对心脏的结构和功能影响已经得到了很好的证实，但运动期间和运动后心脏中发生的代谢变化仍然不清楚。在这项研究中，我们使用代谢组学来评估小鼠心脏代谢组学在1次跑步机运动后的时间依赖性变化。运动结束后，我们还记录了血乳酸、葡萄糖和酮体水平，并测量了心脏线粒体呼吸。在雄性和雌性小鼠中，中等强度和高强度的运动会急剧增加血液乳酸水平。在两种性别中，低强度和中等强度的运动增加循环3-羟基丁酸水平后立即运动回合，然而，只有在雌性小鼠中，高强度运动增加3-羟基丁酸水平，与显着增加发生1小时后的运动会话。对久坐的女性和男性心脏的非靶向代谢组学分析表明，基础心脏代谢物水平存在相当大的性别依赖性差异，女性心脏的特征是泛酸、吡哆胺、高精氨酸、色氨酸和几种甘油磷脂和鞘磷脂物质的水平较高，而许多代谢物的水平较低，包括乙酰辅酶A、葡萄糖醛酸、古洛糖酸、羟脯氨酸、脯氨酰-羟脯氨酸、肌肽、鹅肌肽、和肉毒碱化和甘氨酸化的物种，与男性的心脏相比。在一次跑步机运动后，雄性和雌性小鼠的心脏都有更高水平的皮质酮;然而，雌性小鼠在心脏代谢组中表现出更广泛的运动诱导的变化，其特征在于氨基酸的显著的时间依赖性变化（例如，丝氨酸、丙氨酸、酪氨酸、色氨酸、支链氨基酸）和酮体3-羟基丁酸酯。使用离体心肌线粒体的实验结果表明，高强度跑步机运动不会严重影响呼吸或线粒体偶联;然而，女性心肌线粒体表现出比男性心肌线粒体更高的腺苷二磷酸敏感性。总的来说，这些在小鼠中的发现揭示了心脏代谢的关键性别依赖性差异，并表明女性心脏中的代谢网络对运动引起的生理应激反应更敏感。
Biological sex has considerable influence on cardiac metabolite abundances. Female hearts show more exercise-induced metabolite changes than male hearts. Exercise-induced changes in cardiac metabolites return to baseline within 24 hours. Exercise does not affect cardiac mitochondrial respiration in isolated organelles. Female hearts might have greater intrinsic adenosine diphosphate sensitivity than male hearts. Although the structural and functional effects of exercise on the heart are well established, the metabolic changes that occur in the heart during and after exercise remain unclear. In this study, we used metabolomics to assess time-dependent changes in the murine cardiac metabolome following 1 session of treadmill exercise. After the exercise bout, we also recorded blood lactate, glucose, and ketone body levels and measured cardiac mitochondrial respiration. In both male and female mice, moderate- and high-intensity exercise acutely increased blood lactate levels. In both sexes, low- and moderate-intensity exercise augmented circulating 3-hydroxybutryrate levels immediately after the exercise bout; however, only in female mice did high-intensity exercise increase 3-hydroxybutyrate levels, with significant increases occurring 1 h after the exercise session. Untargeted metabolomics analyses of sedentary female and male hearts suggest considerable sex-dependent differences in basal cardiac metabolite levels, with female hearts characterized by higher levels of pantothenate, pyridoxamine, homoarginine, tryptophan, and several glycerophospholipid and sphingomyelin species and lower levels of numerous metabolites, including acetyl coenzyme A, glucuronate, gulonate, hydroxyproline, prolyl-hydroxyproline, carnosine, anserine, and carnitinylated and glycinated species, as compared with male hearts. Immediately after a bout of treadmill exercise, both male and female hearts had higher levels of corticosterone; however, female mice showed more extensive exercise-induced changes in the cardiac metabolome, characterized by significant, time-dependent changes in amino acids (e.g., serine, alanine, tyrosine, tryptophan, branched-chain amino acids) and the ketone body 3-hydroxybutyrate. Results from experiments using isolated cardiac mitochondria suggest that high-intensity treadmill exercise does not acutely affect respiration or mitochondrial coupling; however, female cardiac mitochondria demonstrate generally higher adenosine diphosphate sensitivity compared with male cardiac mitochondria. Collectively, these findings in mice reveal key sex-dependent differences in cardiac metabolism and suggest that the metabolic network in the female heart is more responsive to physiological stress caused by exercise.
登录
查看更多内容
影响因子：
11.4
作者：
Done AJ;Traustadóttir T
通讯作者：
Traustadóttir T
影响因子：
5.6
作者：
Beikoghli Kalkhoran S;Kararigas G
通讯作者：
Kararigas G
DOI：
10.1111/j.2517-6161.1995.tb02031.x
发表时间：
1995-01-01
影响因子：
5.8
作者：
BENJAMINI, Y;HOCHBERG, Y
通讯作者：
HOCHBERG, Y
影响因子：
64.5
作者：
Boström P;Mann N;Wu J;Quintero PA;Plovie ER;Panáková D;Gupta RK;Xiao C;MacRae CA;Rosenzweig A;Spiegelman BM
通讯作者：
Spiegelman BM
DOI：
10.1249/00005768-199006000-00003
发表时间：
1990-06-01
期刊：
MEDICINE AND SCIENCE IN SPORTS AND EXERCISE
影响因子：
--
作者：
BUNT, JC
通讯作者：
BUNT, JC