Adoptive transfer of suppression of arthritis in the mouse model of collagen-induced arthritis. Evidence for a type II collagen-specific suppressor T cell.
Adoptive transfer of suppression of arthritis in the mouse model of collagen-induced arthritis. Evidence for a type II collagen-specific suppressor T cell.
复制标题
在胶原诱导的关节炎小鼠模型中过继转移关节炎抑制作用。
DOI：
10.1172/jci111917
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发表时间：
1985
期刊：
影响因子：
--
通讯作者：
Moskowitz,RW
中科院分区：
文献类型：
--
作者：
Kresina,TF;Moskowitz,RW
本研究详细介绍了抗原特异性抑制胶原诱导的关节炎的抑制机制。静脉注射500微克的可溶性天然II型胶原蛋白3天前与天然II型胶原蛋白乳化在完全弗氏佐剂的免疫接种导致动物在体外细胞和体液免疫反应降低天然和变性II型胶原蛋白与对照组相比。对照组由预先接种生理盐水和I型胶原蛋白的动物组成，并建立了所观察到的抑制的抗原特异性。还观察到对II型胶原免疫应答降低的小鼠表现出很少或没有与胶原诱导的关节炎相关的红斑和水肿。连续转移实验确立了抑制胶原诱导的关节炎对T细胞的需求。在慢性免疫治疗抑制小鼠在体外反应脾细胞的表型分析表明，反应细胞是Ly 1 -2+（抑制/细胞毒性）T细胞。另一方面，在非抑制型胶原诱导的关节炎小鼠中，对II型胶原应答的T细胞的细胞表型为Ly 1 +2-（辅助/诱导T细胞）。数据表明，II型胶原特异性T细胞在静脉内接种可溶性天然II型胶原时产生。在II型胶原免疫动物中观察到这些细胞，这些动物是非关节炎的，并且描绘了对II型胶原的降低的体液和体外细胞免疫应答。这项研究表明，T抑制细胞对II型胶原免疫反应的特异性抑制可以在某些形式的关节炎中发挥免疫抑制作用。图片
This study details the suppressive mechanism involved in the antigen-specific suppression of collagen-induced arthritis. Intravenous injection of 500 micrograms of soluble native type II collagen 3 d before immunization with native type II collagen emulsified in complete Freund's adjuvant resulted in animals with decreased in vitro cellular and humoral immune response to native and denatured type II collagen compared with control groups. Control groups were composed of animals preinoculated with saline and type I collagen and established the antigen-specific nature of the observed suppression. Mice with reduced immune responses to type II collagen also were observed to portray little or no erythema and edema associated with collagen-induced arthritis. Adoptive transfer experiments established the requirement of T cells for the suppression of collagen-induced arthritis. Analysis of the phenotype of responding splenic cells in chronic immunotherapeutically suppressed mice in vitro revealed that responding cells were Ly1-2+ (suppressor/cytotoxic) T cells. On the other hand, the cellular phenotype of T cells responding to type II collagen in nonsuppressed collagen-induced arthritic mice was Ly1+2- (helper/inducer T cells). The data indicate that type II collagen-specific T cells are generated on intravenous inoculation of soluble native type II collagen. These cells are observed in type II collagen-immune animals, which are nonarthritic and portray reduced humoral and in vitro cellular immune response to type II collagen. This study suggests that specific suppression of immune responses to type II collagen by T-suppressor cells can be immunotherapeutic in certain forms of arthritis.Images
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实验性免疫滑膜炎中血清抗胶原分子的精细特异性。
影响因子：
27.4
作者：
Kresina,TF;Rosner,IA;Goldberg,VM;Moskowitz,RW
通讯作者：
Moskowitz,RW
小鼠 II 型胶原诱导的关节炎
DOI：
--
发表时间：
--
期刊：
影响因子：
--
作者：
J. Stuart;A. Townes;A. Kang
通讯作者：
A. Kang
由抗原脉冲的骨髓源性巨噬细胞诱导的迟发型超敏反应
影响因子：
5.4
作者：
P. Mottram;Jacques F. Miller
通讯作者：
Jacques F. Miller
DOI：
10.1084/jem.141.3.635
发表时间：
1975-03-01
期刊：
The Journal of experimental medicine
影响因子：
--
作者：
Benjamin DC
通讯作者：
Benjamin DC
T细胞子集由Lyt-1,2,3和THY-1抗原的表达定义。单克隆抗体的两参数免疫荧光和细胞毒性分析可修饰当前视图。
DOI：
10.1084/jem.152.2.280
发表时间：
1980-08-01
期刊：
The Journal of experimental medicine
影响因子：
--
作者：
Ledbetter JA;Rouse RV;Micklem HS;Herzenberg LA
通讯作者：
Herzenberg LA