Synergistic delivery of bFGF and BMP-2 from poly(l-lactic-co-glycolic acid)/graphene oxide/hydroxyapatite nanofibre scaffolds for bone tissue engineering applications.
Synergistic delivery of bFGF and BMP-2 from poly(l-lactic-co-glycolic acid)/graphene oxide/hydroxyapatite nanofibre scaffolds for bone tissue engineering applications.
复制标题
DOI：
10.1039/c8ra05250f
复制
发表时间：
2018-09-12
期刊：
影响因子：
3.9
通讯作者：
Zhao, Yan
中科院分区：
文献类型：
--
作者：
Ren, Xiansheng;Liu, Qinyi;Zheng, Shuang;Zhu, Jiaqi;Qi, Zhiping;Fu, Chuan;Yang, Xiaoyu;Zhao, Yan
骨组织工程的目标之一是制备具有良好生物相容性、骨诱导能力和力学性能的支架材料。生物活性蛋白质如骨形态发生蛋白（BMP）-2和碱性成纤维细胞生长因子（bFGF）的应用是提高骨支架材料成骨诱导性和生物相容性的有效途径。因此，开发能够递送多种生长因子的新型材料对于骨缺损修复是迫切和必要的。在这项研究中，一个复合纳米纤维支架组成的聚（l-乳酸-共-乙醇酸）（PLGA），羟基磷灰石（HA），和氧化石墨烯（GO）已被制造提供碱性成纤维细胞生长因子（bFGF）和骨形态发生蛋白-2（BMP-2）的同时。数据显示，将GO和HA掺入PLGA纳米纤维中显著改善了纳米纤维支架的机械性能和亲水性。更重要的是，与PLGA和PLGA/HA纳米纤维支架相比，PLGA/HA/GO纳米纤维支架可以更有效地包裹bFGF和BMP-2。此外，生物学检测表明，负载在复合纳米纤维支架中的bFGF和BMP-2对MC 3 T3-E1细胞的细胞粘附、增殖和成骨分化具有协同分化作用。与PLGA/HA/GO/bFGF和PLGA/HA/GO/BMP-2纳米纤维支架相比，PLGA/HA/GO/bFGF/BMP-2支架显示出更高的ALP活性和更高的成骨相关基因表达水平。总之，我们的研究结果表明，将GO掺入纳米纤维支架中是将生长因子固定到生物材料表面上的有效方法，BMP-2和bFGF的组合的协同效应可能在骨再生治疗中具有潜在的用途。骨组织工程的目标之一是制备具有良好生物相容性、骨诱导能力和力学性能的支架材料。
One of the goals of bone tissue engineering is to create scaffolds with excellent biocompatibility, osteoinductive ability and mechanical properties. The application of bioactive proteins, such as bone morphogenetic protein (BMP)-2 and basic fibroblast growth factor (bFGF), has been showed to be an effective way to improve the osteoinductivity and biocompatibility of bone scaffold materials. Therefore, the development of novel materials capable of delivering multiple growth factors is urgent and essential for bone defect repair. In this study, a composite nanofibre scaffold composed of poly(l-lactic-co-glycolic acid) (PLGA), hydroxyapatite (HA), and graphene oxide (GO) has been fabricated to deliver basic fibroblast growth factor (bFGF) and bone morphogenetic protein-2 (BMP-2) simultaneously. The data show that the incorporation of GO and HA into PLGA nanofibres significantly improved the mechanical properties and hydrophilicity of the nanofibre scaffolds. More importantly, compared to PLGA and PLGA/HA nanofibre scaffolds, the PLGA/HA/GO nanofibre scaffolds could more efficiently immobilize bFGF and BMP-2. Moreover, biological assays indicated that the loaded bFGF and BMP-2 loaded in the composite nanofibre scaffolds have a synergistic differentiation effect on the cell adhesion, proliferation, and osteogenesis differentiation of MC3T3-E1 cells. In contrast to the PLGA/HA/GO/bFGF and PLGA/HA/GO/BMP-2 nanofibre scaffolds, the PLGA/HA/GO/bFGF/BMP-2 scaffolds have shown higher ALP activity and higher expression levels of osteogenesis-related genes. In summary, our findings indicated that the incorporation of GO into nanofibre scaffolds is an effective method to immobilize growth factors onto biomaterial surfaces, and the synergistic effects of a combination of BMP-2 and bFGF may have potential use in bone regenerative therapeutics. One of the goals of bone tissue engineering is to create scaffolds with excellent biocompatibility, osteoinductive ability and mechanical properties.
登录
查看更多内容
影响因子：
--
作者：
Liu, Mengting;Hao, Liying;Cai, Xiaoxiao
通讯作者：
Cai, Xiaoxiao
影响因子：
1.7
作者：
Mohammadi, Sepideh;Shafiei, Seyedeh Sara;Solati-Hashjin, Mehran
通讯作者：
Solati-Hashjin, Mehran
影响因子：
14
作者：
Shen, Hong;Hu, Xixue;Wang, Shenguo
通讯作者：
Wang, Shenguo
影响因子：
--
作者：
Park, Suhyun;Heo, Hyun-A;Pyo, Sung-woon
通讯作者：
Pyo, Sung-woon
影响因子：
11.2
作者：
Kim, Sung Eun;Yun, Young-Pil;Choi, Byung-Joon
通讯作者：
Choi, Byung-Joon