OsCAF2 contains two CRM domains and is necessary for chloroplast development in rice
OsCAF2 contains two CRM domains and is necessary for chloroplast development in rice
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OsCAF2 包含两个 CRM 结构域，是水稻叶绿体发育所必需的
DOI：
10.1186/s12870-020-02593-z
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发表时间：
2020-08
影响因子：
5.3
通讯作者：
Qian
中科院分区：
文献类型：
--
作者：
Lan Shen;Qiang Zhang;Zhongwei Wang;Hongling Wen;Guanglian Hu;Deyong Ren;Jiang Hu;Li Zhu;Zhenyu Gao;Guangheng Zhang;Longbiao Guo;Dali Zeng;Qian
背景叶绿体在植物生长发育中起着重要作用。叶绿体基因组含有约20个II组内含子，这些内含子由于核基因编码的蛋白质而被剪接。CAF 2是一种剪接因子，在玉米和拟南芥中被证明可以剪接IIB组内含子，但水稻中的OsCAF 2基因的研究很少，而且OsCAF 2基因对叶绿体发育的影响还不清楚。表型分析表明，OsCAF 2突变体在苗期叶片出现白化病，最终导致植株死亡，而OsCAF 2突变体叶绿体数量减少，叶绿体结构受损。我们推测OsCAF 2可能参与IIA和IIB组内含子的剪接，这与其在A.还有玉米通过酵母双杂交实验，我们发现OsCAF 2的C端区域与OsCRS 2相互作用，形成OsCAF 2-OsCRS 2复合物。此外，OsCAF 2的N端区域与自身相互作用形成同源二聚体。结论本研究进一步加深了对OsCAF 2蛋白的认识，并揭示了OsCAF 2调控水稻叶绿体发育的分子机制。
BackgroundChloroplasts play an important role in plant growth and development. The chloroplast genome contains approximately twenty group II introns that are spliced due to proteins encoded by nuclear genes. CAF2 is one of these splicing factors that has been shown to splice group IIB introns in maize andArabidopsis thaliana.However, the research of theOsCAF2gene in rice is very little, and the effects ofOsCAF2genes on chloroplasts development are not well characterized.ResultsIn this study,oscaf2mutants were obtained by editing theOsCAF2gene in theNipponbarevariety of rice. Phenotypic analysis showed that mutations toOsCAF2led to albino leaves at the seeding stage that eventually caused plant death, andoscaf2mutant plants had fewer chloroplasts and damaged chloroplast structure. We speculated thatOsCAF2might participate in the splicing of group IIA and IIB introns, which differs from its orthologs inA. thalianaand maize. Through yeast two-hybrid experiments, we found that the C-terminal region of OsCAF2 interacted with OsCRS2 and formed an OsCAF2-OsCRS2 complex. In addition, the N-terminal region of OsCAF2 interacted with itself to form homodimers.ConclusionTaken together, this study improved our understanding of the OsCAF2 protein, and revealed additional information about the molecular mechanism of OsCAF2 in regulating of chloroplast development in rice.
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影响因子：
5.3
作者：
通讯作者：
--
影响因子：
6.9
作者：
Liu C;Zhu H;Xing Y;Tan J;Chen X;Zhang J;Peng H;Xie Q;Zhang Z
通讯作者：
Zhang Z
影响因子：
5.6
作者：
Li Y;Zhang J;Li L;Gao L;Xu J;Yang M
通讯作者：
Yang M
DOI：
--
发表时间：
--
期刊：
--
影响因子：
--
作者：
K. Livak;Thomas D. Schmittgen
通讯作者：
K. Livak;Thomas D. Schmittgen
影响因子：
5.6
作者：
Bobik K;Burch-Smith TM
通讯作者：
Burch-Smith TM