Comparative Transcriptome Analyses Provide New Insights into the Evolution of Divergent Thermal Resistance in Two Eel Gobies.
Comparative Transcriptome Analyses Provide New Insights into the Evolution of Divergent Thermal Resistance in Two Eel Gobies.
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比较转录组分析为两种鳗鱼不同热阻的进化提供了新的见解。
DOI：
10.3390/cimb46010012
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发表时间：
2023-12-25
影响因子：
3.1
通讯作者：
中科院分区：
文献类型：
--
作者：
适应潮汐泥滩的热条件总是需要忍受频繁的波动和经常极端的环境温度。基因表达的调控在这些热适应的进化中起着重要作用。为了确定与热适应相关的关键基因调控网络，我们研究了两种具有不同纬度亲和力的滩涂居民（Odontamblyopus lacepedii和O. rebecca）的耐寒能力，以及在冷胁迫下转录组的变化。结果表明，两种虾虎鱼的耐冷能力存在显著差异（最小耐冷温度分别为0.61 °C和9.57 °C）。对冷胁迫下转录组变化的分析揭示了193个差异表达基因，这些基因在所有组织和物种中表现出相似的表达谱，包括几个经典的代谢和昼夜节律分子，如ACOD和CIART，它们可能代表了鳗鱼虾虎鱼的核心冷反应机制。同时，一些基因在耐寒性较强的O. lacepedii表明它们在增强耐寒性和极端热适应中的作用。此外，加权基因共表达网络分析（WGCNA）揭示了包括MYH 11和LIPT 2在内的代谢中心基因的子集在O. lacepedii在暴露于冷应激时的作用，这突出了减少能量消耗在增强鳗鱼虾虎鱼的耐冷性中的作用。这些发现不仅为泥滩硬骨鱼如何科普冷胁迫及其适应热环境的潜在进化策略提供了新的见解，而且对在全球气候变暖的情况下海洋渔业资源的合理管理和保护具有重要意义。
Adaptation to thermal conditions in tidal mudflats always involves tolerating frequent fluctuations and often extreme environmental temperatures. Regulation of gene expression plays a fundamental role in the evolution of these thermal adaptations. To identify the key gene regulatory networks associated with the thermal adaptation, we investigated the capability of cold tolerance, as well as the transcriptomic changes under cold stress in two mudflat inhabitants (Odontamblyopus lacepedii and O. rebecca) with contrasting latitude affinity. Our results revealed a remarkable divergent capacity of cold tolerance (CTmin: 0.61 °C vs. 9.57 °C) between the two gobies. Analysis of transcriptomic changes under cold stress unveiled 193 differentially expressed genes exhibiting similar expression profiles across all tissues and species, including several classic metabolic and circadian rhythm molecules such as ACOD and CIART that may represent the core cold response machinery in eel gobies. Meanwhile, some genes show a unique expression spectrum in the more cold-tolerant O. lacepedii suggesting their roles in the enhanced cold tolerance and hence the extreme thermal adaptations. In addition, a weighted gene co-expression network analysis (WGCNA) revealed a subset of metabolic hub genes including MYH11 and LIPT2 showing distinct down-regulation in O. lacepedii when exposed to cold stress which highlights the role of reduced energy consumption in the enhanced cold tolerance of eel gobies. These findings not only provide new insights into how mudflat teleosts could cope with cold stress and their potential evolutionary strategies for adapting to their thermal environment, but also have important implications for sound management and conservation of their fishery resources in a scenario of global climate warming in the marine realm.
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影响因子：
10.7
作者：
Jiang, Haifeng;Lv, Wenqi;Wang, Ying;Qian, Yuting;Wang, Cheng;Sun, Ning;Fang, Chengchi;Irwin, David M.;Gan, Xiaoni;He, Shunping;Yang, Liandong
通讯作者：
Yang, Liandong
WGCNA：用于加权相关网络分析的 R 包。
影响因子：
3
作者：
Langfelder P;Horvath S
通讯作者：
Horvath S
影响因子：
2.2
作者：
Li，Yuan-Jie;Ma，Chun-Sen;Colinet，Herve
通讯作者：
Colinet，Herve
影响因子：
1.4
作者：
BECKER, CD;GENOWAY, RG
通讯作者：
GENOWAY, RG
DOI：
10.1111/j.2517-6161.1995.tb02031.x
发表时间：
1995-01-01
影响因子：
5.8
作者：
BENJAMINI, Y;HOCHBERG, Y
通讯作者：
HOCHBERG, Y