Permethrin alters glucose metabolism in conjunction with high fat diet by potentiating insulin resistance and decreases voluntary activities in female C57BL/6J mice.
Permethrin alters glucose metabolism in conjunction with high fat diet by potentiating insulin resistance and decreases voluntary activities in female C57BL/6J mice.
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DOI：
10.1016/j.fct.2017.07.053
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发表时间：
2017-10
期刊：
影响因子：
--
通讯作者：
Park Y
中科院分区：
文献类型：
--
作者：
Xiao X;Kim Y;Kim D;Yoon KS;Clark JM;Park Y
氯菊酯是一种1型拟除虫菊酯类杀虫剂，以前曾报道可促进3 T3-L1脂肪细胞中的脂肪生成和C2 C12肌细胞中的胰岛素抵抗;然而，氯菊酯暴露对体内葡萄糖和脂质代谢的影响尚不清楚。本研究的目的是探讨氯菊酯暴露对雌性小鼠血糖和血脂稳态以及自主运动的影响。我们在低脂肪饮食（饮食的4%w/w）和高脂肪饮食（饮食的20%w/w）的雌性C57 BL/6 J小鼠中测试了三种剂量的氯菊酯（50、500和5000 μg/kg体重/天），持续12周。我们的研究结果表明，氯菊酯治疗增强高脂饮食诱导的胰岛素抵抗，如胰岛素耐量试验，葡萄糖耐量试验，和稳态模型评估-胰岛素抵抗（HOMA-IR），而不改变体重或脂肪量。氯菊酯治疗显着减少自愿运动和血糖和胰岛素水平升高。蛋白质印迹结果进一步表明，氯菊酯通过腓肠肌中的Akt信号通路损害胰岛素信号传导。总之，这些结果表明，口服氯菊酯增强高脂饮食诱导的胰岛素抵抗，可能增加2型糖尿病的风险，而不改变雌性C57 BL/6 J小鼠的体重增加。
Permethrin, a type 1 pyrethroid insecticide, was previously reported to promote adipogenesis in 3T3-L1 adipocytes and insulin resistance in C2C12 muscle cells; however, the effects of permethrin exposure on glucose and lipid metabolisms in vivo remain unknown. The purpose of this study was to investigate the effects of permethrin exposure on glucose and lipid homeostasis as well as voluntary movement in female mice in response to dietary fat. We tested three doses of permethrin (50, 500, & 5000 μg/kg body weight/day) in low fat diet-fed (4% w/w of diet) and high fat diet-fed (20% w/w of diet) female C57BL/6J mice for twelve weeks. Our results demonstrated that permethrin treatment potentiated high fat diet-induced insulin resistance as indicated by insulin tolerance tests, glucose tolerance tests, and homeostasis model assessment - insulin resistance (HOMA-IR) without altering weight or fat mass. Permethrin treatment significantly decreased voluntary movement and elevated blood glucose and insulin levels. Western blot results further showed that permethrin impaired insulin signaling via the Akt signaling pathway in the gastrocnemius muscle. Taken together, these results suggest that oral administration of permethrin potentiated high fat diet-induced insulin resistance, possibly increasing the risk of type 2 diabetes without altering weight gain in female C57BL/6J mice.
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影响因子：
10.4
作者：
Cox S;Niskar AS;Narayan KM;Marcus M
通讯作者：
Marcus M
影响因子：
10.4
作者：
Lassiter, T. Leon;Ryde, Ian T.;MacKillop, Emiko A.;Brown, Kathleen K.;Levin, Edward D.;Seidler, Frederic J.;Slotkin, Theodore A.
通讯作者：
Slotkin, Theodore A.
影响因子：
3.8
作者：
ANADON, A;MARTINEZLARRANAGA, MR;BRINGAS, P
通讯作者：
BRINGAS, P
影响因子：
3.6
作者：
Kim, Jonggun;Park, Yooheon;Park, Yeonhwa
通讯作者：
Park, Yeonhwa
影响因子：
8.2
作者：
Lee, D.-H.;Lee, I.-K.;Jacobs, D. R., Jr.
通讯作者：
Jacobs, D. R., Jr.