Wheat genetic loci conferring resistance to stripe rust in the face of genetically diverse races of the fungus Puccinia striiformis f. sp. tritici.
Wheat genetic loci conferring resistance to stripe rust in the face of genetically diverse races of the fungus Puccinia striiformis f. sp. tritici.
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DOI：
10.1007/s00122-021-03967-z
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发表时间：
2022-01
期刊：
影响因子：
--
通讯作者：
Cockram J
中科院分区：
文献类型：
--
作者：
Bouvet L;Percival-Alwyn L;Berry S;Fenwick P;Mantello CC;Sharma R;Holdgate S;Mackay IJ;Cockram J
对小麦多创始群体进行分析，确定了14个抗条锈病QTL。单倍型分析表明，在四个最显著的QTL中，有三个在欧洲小麦中是罕见的抗性等位基因。条锈病是由条锈菌(Puccinia striiformis Westend f.sp.)引起的小麦(Triticum Aestivum)的一种主要真菌病害。小麦(PST)。自2011年以来，历史上克隆的欧洲PST小种已被遗传多样性谱系的快速入侵所取代，降低了以前表现出持久抗性的品种的抗性。鉴定这些小种的遗传抗性来源是小麦育种的首要任务。在这里，我们使用一个具有90,000个特征单核苷酸多态阵列的小麦八个创建者多亲本群体来遗传定位这些新的PST小种的YR抗性。对英国3个地点的5个田间试验进行的遗传分析确定了14个与抗性有关的数量性状基因座(QTL)。其中，在所有测试环境中一致地发现了4个高度显著的基因座，分别位于染色体1A(QYr.niab-1A.1)、2A(QYr.niab-2A.1)、2B(QYr.niab-2B.1)和2D(QYr.niab-2D.1)上，共同解释了 ~ 50%的表型变异。对这4个QTL的双向和三向组合分析表明，组合比单个QTL具有更强的抗性，并开发了区分抗性和感病等位基因的遗传标记。对一系列小麦品种的单倍型分析发现，这四个主要基因座中的三个与YR抗性相关的单倍型在欧洲小麦中是罕见的(≤ 7%)，这突显了它们在未来有针对性地提高抗病性方面的潜在用途。值得注意的是，QTL QYr.niab-2B.1的物理区间包含5个核苷酸结合的富亮氨酸重复候选基因，其中2个对应于克隆的抗性基因yr7和Yr5/YrSp。网上版载有补充材料，可在10.1007/s00122-021-03967-z查阅。
Analysis of a wheat multi-founder population identified 14 yellow rust resistance QTL. For three of the four most significant QTL, haplotype analysis indicated resistance alleles were rare in European wheat. Stripe rust, or yellow rust (YR), is a major fungal disease of wheat (Triticum aestivum) caused by Puccinia striiformis Westend f. sp. tritici (Pst). Since 2011, the historically clonal European Pst races have been superseded by the rapid incursion of genetically diverse lineages, reducing the resistance of varieties previously showing durable resistance. Identification of sources of genetic resistance to such races is a high priority for wheat breeding. Here we use a wheat eight-founder multi-parent population genotyped with a 90,000 feature single nucleotide polymorphism array to genetically map YR resistance to such new Pst races. Genetic analysis of five field trials at three UK sites identified 14 quantitative trait loci (QTL) conferring resistance. Of these, four highly significant loci were consistently identified across all test environments, located on chromosomes 1A (QYr.niab-1A.1), 2A (QYr.niab-2A.1), 2B (QYr.niab-2B.1) and 2D (QYr.niab-2D.1), together explaining ~ 50% of the phenotypic variation. Analysis of these four QTL in two-way and three-way combinations showed combinations conferred greater resistance than single QTL, and genetic markers were developed that distinguished resistant and susceptible alleles. Haplotype analysis in a collection of wheat varieties found that the haplotypes associated with YR resistance at three of these four major loci were rare (≤ 7%) in European wheat, highlighting their potential utility for future targeted improvement of disease resistance. Notably, the physical interval for QTL QYr.niab-2B.1 contained five nucleotide-binding leucine-rich repeat candidate genes with integrated BED domains, of which two corresponded to the cloned resistance genes Yr7 and Yr5/YrSp. The online version contains supplementary material available at 10.1007/s00122-021-03967-z.
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影响因子：
12.3
作者：
Alaux M;Rogers J;Letellier T;Flores R;Alfama F;Pommier C;Mohellibi N;Durand S;Kimmel E;Michotey C;Guerche C;Loaec M;Lainé M;Steinbach D;Choulet F;Rimbert H;Leroy P;Guilhot N;Salse J;Feuillet C;International Wheat Genome Sequencing Consortium;Paux E;Eversole K;Adam-Blondon AF;Quesneville H
通讯作者：
Quesneville H
影响因子：
5.6
作者：
Ali S;Rodriguez-Algaba J;Thach T;Sørensen CK;Hansen JG;Lassen P;Nazari K;Hodson DP;Justesen AF;Hovmøller MS
通讯作者：
Hovmøller MS
影响因子：
3.3
作者：
Bueno-Sancho V;Persoons A;Hubbard A;Cabrera-Quio LE;Lewis CM;Corredor-Moreno P;Bunting DCE;Ali S;Chng S;Hodson DP;Madariaga Burrows R;Bryson R;Thomas J;Holdgate S;Saunders DGO
通讯作者：
Saunders DGO
影响因子：
3.6
作者：
Axtell, MJ;Chisholm, ST;Staskawicz, BJ
通讯作者：
Staskawicz, BJ
影响因子：
5.6
作者：
Downie RC;Bouvet L;Furuki E;Gosman N;Gardner KA;Mackay IJ;Campos Mantello C;Mellers G;Phan HTT;Rose GA;Tan KC;Oliver RP;Cockram J
通讯作者：
Cockram J