Assessing European Wheat Sensitivities to Parastagonospora nodorum Necrotrophic Effectors and Fine-Mapping the Snn3-B1 Locus Conferring Sensitivity to the Effector SnTox3.
Assessing European Wheat Sensitivities to Parastagonospora nodorum Necrotrophic Effectors and Fine-Mapping the Snn3-B1 Locus Conferring Sensitivity to the Effector SnTox3.
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DOI：
10.3389/fpls.2018.00881
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发表时间：
2018
影响因子：
5.6
通讯作者：
Cockram J
中科院分区：
文献类型：
--
作者：
Downie RC;Bouvet L;Furuki E;Gosman N;Gardner KA;Mackay IJ;Campos Mantello C;Mellers G;Phan HTT;Rose GA;Tan KC;Oliver RP;Cockram J
小麦(Triticum aestivum L.)是世界上最重要的农作物之一，其病原菌是小麦的一种坏死性真菌。P.nodorum利用蛋白质类的坏死营养效应器来调节宿主细胞的死亡，推测释放出的营养物质使感染过程得以继续。病原体效应物的识别使得寄主的遗传抗性机制能够被分离成它们的组成部分。在P.nodorum中，已经克隆了三个蛋白质效应子：SnToxA、SnTox1和SnTox3。在这里，我们调查了480个欧洲小麦品种对所有三个效应因子的敏感性，并使用全基因组联合扫描(GWAS)和一个由八个创始人组成的小麦多亲本高级世代间杂交(MAGIC)群体精细定位了小麦SnTox3敏感基因座Snn3-B1。使用Bonferroni校正的Plog0.05显著阈值，GWAs确定了10个显著标记，该标记定义了位于5B染色体短臂上的单个基因座≤3-B1，可解释32%的表型变异[峰值单核苷酸多态(SNPs)，Excalbur_c47452183和基因-3324338，-log10P=20.44]。对魔术群体中SnTox3敏感性的单标记分析通过5个显著的SNP定位了Snn3-B1，定义了一个6.2kb的区域，其中包括在关联图谱小组中发现的两个峰值SNP。因此，将该SNP转化为KASP基因分型系统，并通过对95个小麦品种亚集的筛选验证，为分子标记辅助育种和进一步的遗传研究提供了有价值的资源。此外，在魔术群体中发现了6个微小的SnTox3敏感数量性状基因座，分别位于第2A(QTox3.niab-2A.1，P=9.17-7)、2B(QTox3.niab-2B.1，P=0.018)、3B(QTox3.niab-3B.1，P=48.51-4)、4D(QTox3.niab-4D.1，P=0.028)、6A(QTox3.niab-6A.1，P=8.51-4)和7B(QTox3.niab-7B.1，P=0.020)上。各性状对表型变异的贡献率在3.1-6.0%之间。总之，本研究的结果为育种家提供了关于欧洲小麦种质对P.nodorum效应子的敏感性的知识和资源，以及用于确定Snn3-B1等位基因状态的简单诊断标记。
Parastagonospora nodorum is a necrotrophic fungal pathogen of wheat (Triticum aestivum L.), one of the world’s most important crops. P. nodorum mediates host cell death using proteinaceous necrotrophic effectors, presumably liberating nutrients that allow the infection process to continue. The identification of pathogen effectors has allowed host genetic resistance mechanisms to be separated into their constituent parts. In P. nodorum, three proteinaceous effectors have been cloned: SnToxA, SnTox1, and SnTox3. Here, we survey sensitivity to all three effectors in a panel of 480 European wheat varieties, and fine-map the wheat SnTox3 sensitivity locus Snn3-B1 using genome-wide association scans (GWAS) and an eight-founder wheat multi-parent advanced generation inter-cross (MAGIC) population. Using a Bonferroni corrected P ≤ 0.05 significance threshold, GWAS identified 10 significant markers defining a single locus, Snn3-B1, located on the short arm of chromosome 5B explaining 32% of the phenotypic variation [peak single nucleotide polymorphisms (SNPs), Excalibur_c47452_183 and GENE-3324_338, -log10P = 20.44]. Single marker analysis of SnTox3 sensitivity in the MAGIC population located Snn3-B1 via five significant SNPs, defining a 6.2-kb region that included the two peak SNPs identified in the association mapping panel. Accordingly, SNP Excalibur_c47452_183 was converted to the KASP genotyping system, and validated by screening a subset of 95 wheat varieties, providing a valuable resource for marker assisted breeding and for further genetic investigation. In addition, composite interval mapping in the MAGIC population identified six minor SnTox3 sensitivity quantitative trait loci, on chromosomes 2A (QTox3.niab-2A.1, P-value = 9.17-7), 2B (QTox3.niab-2B.1, P = 0.018), 3B (QTox3.niab-3B.1, P = 48.51-4), 4D (QTox3.niab-4D.1, P = 0.028), 6A (QTox3.niab-6A.1, P = 8.51-4), and 7B (QTox3.niab-7B.1, P = 0.020), each accounting for between 3.1 and 6.0 % of the phenotypic variance. Collectively, the outcomes of this study provides breeders with knowledge and resources regarding the sensitivity of European wheat germplasm to P. nodorum effectors, as well as simple diagnostic markers for determining allelic state at Snn3-B1.
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DOI：
10.1534/g3.115.021584
发表时间：
2015-09-28
期刊：
G3 (Bethesda, Md.)
影响因子：
--
作者：
Cockram J;Scuderi A;Barber T;Furuki E;Gardner KA;Gosman N;Kowalczyk R;Phan HP;Rose GA;Tan KC;Oliver RP;Mackay IJ
通讯作者：
Mackay IJ
影响因子：
6.7
作者：
Liu Z;Zhang Z;Faris JD;Oliver RP;Syme R;McDonald MC;McDonald BA;Solomon PS;Lu S;Shelver WL;Xu S;Friesen TL
通讯作者：
Friesen TL
影响因子：
2.7
作者：
BALLANCE, GM;LAMARI, L;BERNIER, CC
通讯作者：
BERNIER, CC
影响因子：
5.8
作者：
Huang, B. Emma;George, Andrew W.
通讯作者：
George, Andrew W.
影响因子：
7.4
作者：
Friesen, Timothy L.;Zhang, Zengcui;Faris, Justin D.
通讯作者：
Faris, Justin D.