Marker-assisted development and characterization of near-isogenic lines carrying the Rx4 gene for hypersensitive resistance to Xanthomonas euvesicatoria pv. perforans race T3 in tomato
Marker-assisted development and characterization of near-isogenic lines carrying the Rx4 gene for hypersensitive resistance to Xanthomonas euvesicatoria pv. perforans race T3 in tomato
复制标题
携带 Rx4 基因的近等基因系的标记辅助开发和表征，对 Xanthomonas euvesicatoria pv. 具有超敏抗性。
DOI：
10.1007/s11032-019-1084-2
复制
发表时间：
2019-12
影响因子：
3.1
通讯作者：
Yang Wencai
中科院分区：
文献类型：
--
作者：
Li Ning;Zhang Xiaofei;Yang Wencai
由几种黄单胞菌引起的细菌性斑点病是一种毁灭性的疾病，导致全世界番茄产区严重的果实产量损失和质量下降。缺乏抗性商业品种是限制应用综合管理控制该病害的最关键因素之一。尽管多年来已经发现了对所有物种和种族的抗性来源，但抗该病的育种进展缓慢，因为抗性是数量遗传的，有时与不良的园艺性状有关。通过将供体抗性基因区域的最短 DNA 片段渗入优良品种来开发近等基因系 (NIL)，是消除对园艺性状负面影响的一种有前景的方法。本研究采用传统回交与分子标记辅助前景和背景选择相结合的策略来产生携带Rx4基因的NILs，用于抗黄单胞菌pv.perforansrace T3菌株。供体亲本是野生品种Solanum pimpinellifolium accession PI 128216，轮回亲本是优良加工番茄育种系OH88119。通过将 T3 菌株 Xv829 渗入植物叶片，在 F1、BC4F1、BC6F1、BC6F2、BC6F3 和 BC6F4 代中进行疾病评估。同时，通过重复田间试验，收集了 BC6F4 和 BC6F5 的果实性状，包括果实重量、果实颜色参数、可溶性固形物含量、pH 值和硬度。获得了 Rx4 基因区域中具有 159-1901 kb DNA 片段的 5 个 NIL。它们均对T3小种表现出高水平的过敏抗性，其果实性状与轮回亲本OH88119没有显着差异。然而，在 Rx4 基因区域携带最大 1901 kb DNA 片段的 NIL Rx4–1812 的果实重量比 OH8819 低 9.5–11.8%，尽管差异不具有统计学意义。这可能是由于FW11.2（同一地区的果实重量QTL）的连锁拖累所致。总体而言，这里创建的 NIL 可用作开发抗性品种和破译番茄细菌性斑点病抗性机制的来源。
Bacterial spot caused by several species ofXanthomonasis a devastating disease resulting in severe fruit yield loss and quality decline in tomato production areas worldwide. Lack of resistant commercial varieties is among the most critical factors limiting the application of integrated management to control the disease. Although sources for resistance to all species and races have been discovered over years, breeding for resistance to the disease is slow because the resistance is quantitatively inherited and sometimes linked to poor horticultural traits. Development of near-isogenic lines (NILs) by introgressing the shortest DNA fragment of the resistance gene region from the donor into elite cultivars is a promising approach to eliminating the negative effect on horticultural traits. In the current study, a strategy of combining traditional backcross with molecular marker-assisted foreground and background selection was adopted to generate NILs carrying theRx4gene for resistance toXanthomonas euvesicatoriapv.perforansrace T3 strain. The donor parent is a wild speciesSolanum pimpinellifoliumaccession PI 128216, and the recurrent parent is an elite processing tomato breeding line OH88119. Disease evaluation was performed in F1, BC4F1, BC6F1, BC6F2, BC6F3,and BC6F4generations by infiltration of race T3 strainXv829to leaves of plants. Meanwhile, fruit traits including fruit weight, fruit color parameters, soluble solid content, pH, and firmness were collected from replicated field trials in BC6F4and BC6F5. Five NILs with 159–1901 kb DNA fragments in theRx4gene region were obtained. They all showed high level of hypersensitive resistance to race T3, and their fruit traits were not significantly different from the recurrent parent OH88119. However, fruit weight of one NIL Rx4–1812 carrying the largest DNA fragment of 1901 kb in theRx4gene region was 9.5–11.8% lower than OH8819 though the difference was not statistically significant. This might be due to the linkage drag ofFW11.2, a QTL for fruit weight in the same region. Overall, the NILs created here can be used as sources for developing resistant varieties and deciphering the mechanism of resistance to bacterial spot in tomato.
登录
查看更多内容
DOI：
10.21273/jashs.128.5.0698
发表时间：
2003-09
影响因子：
1.9
作者：
J. Scott;D. Francis;S. Miller;G. Somodi;Jeffrey B. Jones
通讯作者：
J. Scott;D. Francis;S. Miller;G. Somodi;Jeffrey B. Jones
影响因子：
4.1
作者：
Leandri T Rossouw;N. Madala;F. Tugizimana;P. Steenkamp;L. L. Esterhuizen-L.;I. Dubery
通讯作者：
Leandri T Rossouw;N. Madala;F. Tugizimana;P. Steenkamp;L. L. Esterhuizen-L.;I. Dubery
影响因子：
5.3
作者：
Gao D;Huibers RP;Loonen AE;Visser RG;Wolters AM;Bai Y
通讯作者：
Bai Y
影响因子：
2.3
作者：
J. E. Haggard;D. A. St.Clair
通讯作者：
J. E. Haggard;D. A. St.Clair
影响因子：
4.3
作者：
F. Rubio;A. Alonso;S. García-Martínez;J. J. Ruiz-J.
通讯作者：
F. Rubio;A. Alonso;S. García-Martínez;J. J. Ruiz-J.