Transcriptome Profiling and Analysis of Genes Associated with High Temperature–Induced Masculinization in Sex-Undifferentiated Nile Tilapia Gonad
Transcriptome Profiling and Analysis of Genes Associated with High Temperature–Induced Masculinization in Sex-Undifferentiated Nile Tilapia Gonad
复制标题
性未分化尼罗罗非鱼性腺高温诱导雄性化相关基因的转录组分析
DOI：
10.1007/s10126-020-09956-5
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发表时间：
2020-02
影响因子：
3
通讯作者：
Xiang Shan Ji
中科院分区：
文献类型：
--
作者：
Jian Teng;Yan Zhao;Hong Ju Chen;Hui Wang;Xiang Shan Ji
在关键热敏期（TSPs）人工高温可诱导尼罗罗非鱼（Oreochromis niloticus）雌鱼变性为假雌鱼；尼罗罗非鱼是一种GSD + TE（基因型+温度效应）鱼类。以往的研究表明，水温会影响各种硬骨鱼的性腺或大脑中许多基因的表达水平。然而，关于温度在GSD + TE鱼类性别分化早期全性腺转录组水平上的影响的研究很少报道。在这项研究中，RNA-Seq用于表征转录组谱，并鉴定21 dpf时尼罗罗非鱼性腺中表现出温度和性别偏向表达的基因。共有42个基因被发现与高温处理和性发育相关，因为这些基因在FC（雌性对照）与MC（雄性对照）和FC与FT （TSP中高温处理的雌性）中的表达水平不同。在这些基因中，dmrt1、Gsdf、DNA损伤诱导蛋白GADD45 α等多个雄性性别决定和分化基因的转录改变表明，雄性途径是在高温处理后启动的，其启动可能在尼罗罗非鱼高温诱导的雄性化过程中发挥作用。13个差异表达基因的qRT-PCR验证结果显示，qPCR结果与RNA-Seq结果log10倍变化值的Pearson相关为0.70 (p< 0.001)，说明RNA-Seq结果的准确性和可靠性。我们的研究为高温处理如何诱导尼罗罗非鱼雌性性别逆转提供了见解。
Artificially high temperatures during critical thermosensitive periods (TSPs) can induce the sex reversal of Nile tilapia (Oreochromis niloticus) females into pseudomales; Nile tilapia is a GSD + TE (genotypic plus temperature effects) fish species. Previous studies have shown that water temperature affects the expression levels of many genes in the gonad or brain in various teleost species. However, few studies on the effect of temperature at the whole-gonad transcriptomic level in the early stage of sex differentiation have been reported in fish species exhibiting GSD + TE. In this study, RNA-Seq was performed to characterize the transcriptomic profile and identify genes exhibiting temperature- and sex-biased expressions in the Nile tilapia gonad at 21 dpf. A total of 42 genes were found to be associated with both high-temperature treatment and sex development, as the expression levels of these genes differed in both FC (female control) vs MC (male control) and FC vs FT (high temperature–treated females in the TSP). Among these genes, the transcriptional alterations of many male sex determination and differentiation genes, such asDmrt1,Gsdf, and the DNA damage-inducible protein GADD45 alpha, suggested that the male pathway is initiated after high-temperature treatment and that its initiation may play a role in high temperature–induced masculinization in Nile tilapia. The qRT-PCR validation results for thirteen differentially expressed genes showed that the Pearson’s correlation of the log10fold change values between the qPCR and RNA-Seq results was 0.70 (p< 0.001), indicating the accuracy and reliability of the RNA-Seq results. Our study provides insights into how high-temperature treatment induces the sex reversal of Nile tilapia females.
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影响因子：
3.7
作者：
Ospina-Alvarez N;Piferrer F
通讯作者：
Piferrer F
影响因子：
4.4
作者：
Díaz N;Piferrer F
通讯作者：
Piferrer F
DOI：
10.1016/j.cbpb.2014.08.006
发表时间：
2014-11-01
影响因子：
2.2
作者：
Li, Chun Ge;Wang, Hui;Ji, Xiang Shan
通讯作者：
Ji, Xiang Shan
影响因子：
4.5
作者：
Beardmore, JA;Mair, GC;Lewis, RI
通讯作者：
Lewis, RI
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影响因子：
5.6
作者：
Wei L;Yang C;Tao W;Wang D
通讯作者：
Wang D