Genetic and biochemical analyses of the Pseudomonas aeruginosa Psl exopolysaccharide reveal overlapping roles for polysaccharide synthesis enzymes in Psl and LPS production.
Genetic and biochemical analyses of the Pseudomonas aeruginosa Psl exopolysaccharide reveal overlapping roles for polysaccharide synthesis enzymes in Psl and LPS production.
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DOI：
10.1111/j.1365-2958.2009.06795.x
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发表时间：
2009-08
影响因子：
3.6
通讯作者：
Wozniak DJ
中科院分区：
文献类型：
--
作者：
Byrd MS;Sadovskaya I;Vinogradov E;Lu H;Sprinkle AB;Richardson SH;Ma L;Ralston B;Parsek MR;Anderson EM;Lam JS;Wozniak DJ
胞外多糖对机会致病菌铜绿假单胞菌的粘附和生物膜形成有重要作用。由多糖合成位点（psl）合成的Psl多糖是不依赖藻酸盐作为主要生物膜多糖的非粘液样菌株中生物膜形成所需的。单个psl基因的框内缺失和互补研究揭示了11个psl基因pslACDEFGHIJKL是Psl生产和表面附着所需的。我们还提出了Psl依赖性多糖的第一个结构分析，其由含有d-甘露糖、d-葡萄糖和l-鼠李糖的重复五糖组成：此外，我们鉴定了参与Psl生成的糖核苷酸前体，并证明了Psl生产和表面附着中对GDP-d-甘露糖、UDP-d-葡萄糖和dTDP-l-鼠李糖的需求。最后，遗传分析表明，wbpW恢复Psl生产的pslB突变体和pslB促进A-带LPS合成的wbpW突变体，这两种酶的功能冗余和重叠的作用。本文提供的结构和遗传数据为进一步研究Psl蛋白和Psl在铜绿假单胞菌的生物学和发病机制中的潜在作用提供了基础。
Exopolysaccharides contribute significantly to attachment and biofilm formation in the opportunisitc pathogen Pseudomonas aeruginosa. The Psl polysaccharide, which is synthesized by the polysaccharide synthesis locus (psl), is required for biofilm formation in nonmucoid strains that do not rely on alginate as the principal biofilm polysaccharide. In-frame deletion and complementation studies of individual psl genes revealed that eleven psl genes, pslACDEFGHIJKL, are required for Psl production and surface attachment. We also present the first structural analysis of the psl-dependent polysaccharide, which consists of a repeating pentasaccharide containing d-mannose, d-glucose, and l-rhamnose: In addition we identified the sugar nucleotide precursors involved in Psl generation and demonstrated the requirement for GDP-d-mannose, UDP-d-glucose, and dTDP-l-rhamnose in Psl production and surface attachment. Finally, genetic analyses revealed that wbpW restored Psl production in a pslB mutant and pslB promoted A-band LPS synthesis in a wbpW mutant, indicating functional redundancy and overlapping roles for these two enzymes. The structural and genetic data presented here provide a basis for further investigation of the Psl proteins and potential roles for Psl in the biology and pathogenesis of P. aeruginosa.
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影响因子：
9.4
作者：
LAM, MYC;MCGROARTY, EJ;LAM, JS
通讯作者：
LAM, JS
DOI：
10.1099/00221287-13-3-572
发表时间：
1955-01-01
期刊：
JOURNAL OF GENERAL MICROBIOLOGY
影响因子：
--
作者：
HOLLOWAY, BW
通讯作者：
HOLLOWAY, BW
影响因子：
3.2
作者：
JAYARATNE, P;BRONNER, D;WHITFIELD, C
通讯作者：
WHITFIELD, C
影响因子：
3.1
作者：
HANCOCK, REW;MUTHARIA, LM;PIER, GB
通讯作者：
PIER, GB
影响因子：
3.1
作者：
LAM, JS;MACDONALD, LA;SOUTHAM, GG
通讯作者：
SOUTHAM, GG