The neurotrophin receptor p75 regulates gustatory axon branching and promotes innervation of the tongue during development.
The neurotrophin receptor p75 regulates gustatory axon branching and promotes innervation of the tongue during development.
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DOI：
10.1186/1749-8104-9-15
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发表时间：
2014-06-24
影响因子：
3.6
通讯作者：
Krimm RF
中科院分区：
文献类型：
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作者：
Fei D;Huang T;Krimm RF
脑源性神经营养因子（Brain-derived neurotrophic factor， BDNF）和神经营养因子-4 （neurotrophin-4, NT4）在发育过程中调控味觉神经元的存活、轴突的生长和分支以及味蕾的神经支配。这些作用在很大程度上，但不是完全，通过酪氨酸激酶受体TrkB介导。在这里，我们研究了p75 （BDNF和NT4的另一个主要受体）在味觉系统发育中的作用。我们发现p75−/−小鼠在胚胎日(E)13.5时表现出轴突生长延迟和味觉轴突分支减少。从E14.5到E18.5， p75−/−小鼠的味觉神经元支配的乳头较少，完全不能支配舌中部区域。p75突变对味觉轴突的这些早期影响早于E14.5开始的节状神经节神经元的丧失，也导致了出生时和出生后味蕾的丧失。由于TrkB受体（TrkB−/−）的敲除不像其两个配体（BDNF和NT4）的杂交敲除那样失去那么多的味蕾，我们想知道p75是否在缺乏TrkB的情况下维持了这些额外的味蕾。事实并非如此；TrkB−/−/p75−/−杂交小鼠比TrkB−/−小鼠有更多的味蕾；这些额外的味蕾并不是由于神经元或神经支配的增加。在味觉系统发育过程中，P75调节味觉神经元轴突分支和舌神经支配模式。该功能可能独立于BDNF、NT4和TrkB完成。此外，p75不支持TrkB−/−小鼠中剩余的神经元或味蕾。
Brain-derived neurotrophic factor (BDNF) and neurotrophin-4 (NT4) regulate the survival of gustatory neurons, axon growth and branching, and innervation of taste buds during development. These actions are largely, but not completely, mediated through the tyrosine kinase receptor, TrkB. Here, we investigated the role of p75, the other major receptor for BDNF and NT4, in the development of the taste system. We found that p75−/−mice showed delayed axon outgrowth and reduced branching of gustatory axons at embryonic day (E)13.5. From E14.5 to E18.5, gustatory neurons innervated fewer papillae and completely failed to innervate the mid-region of the tongue in p75−/−mice. These early effects of the p75 mutation on gustatory axons preceded the loss of geniculate ganglion neurons starting at E14.5 and also contributed to a loss of taste buds at and after birth. Because knockouts for the TrkB receptor (TrkB−/−) do not lose as many taste buds as hybrid knockouts for its two ligands (BDNF and NT4), we asked if p75 maintains those additional taste buds in the absence of TrkB. It does not; hybrid TrkB−/−/p75−/−mice had more taste buds than TrkB−/−mice; these additional taste buds were not due to an increase in neurons or innervation. p75 regulates gustatory neuron axon branching and tongue innervation patterns during taste system development. This function is likely accomplished independently of BDNF, NT4, and TrkB. In addition, p75 does not support the remaining neurons or taste buds in TrkB−/−mice.
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味道神经元由大的TRKB受体依赖性和小型TRKB受体独立的亚群组成。
影响因子：
3.7
作者：
Fei D;Krimm RF
通讯作者：
Krimm RF
影响因子：
5.3
作者：
Ben-Zvi, Ayal;Ben-Gigi, Liat;Behar, Oded
通讯作者：
Behar, Oded
影响因子：
2.7
作者：
Krimm, RF;Miller, KK;Albers, KM
通讯作者：
Albers, KM
影响因子：
56.9
作者：
Deppmann, Christopher D.;Mihalas, Stefan;Ginty, David D.
通讯作者：
Ginty, David D.
影响因子：
--
作者：
ABERCROMBIE, M
通讯作者：
ABERCROMBIE, M