Periostin is frequently overexpressed and enhances invasion and angiogenesis in oral cancer.
Periostin is frequently overexpressed and enhances invasion and angiogenesis in oral cancer.
复制标题
骨膜素经常过表达，并增强口腔癌的侵袭和血管生成。
DOI：
10.1038/sj.bjc.6603431
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发表时间：
2006-11-20
影响因子：
8.8
通讯作者：
中科院分区：
文献类型：
--
作者：
口腔鳞状细胞癌（OSCC）是人类最常见的癌症之一。典型的OSCC细胞表现出持续的侵袭，经常导致局部复发和远处淋巴转移。我们先前通过比较亲本OSCC细胞系和高侵袭性克隆的转录谱，将骨膜蛋白鉴定为在侵袭性克隆中表现出最高倍数变化表达的基因。在这里，我们证明了骨膜蛋白过表达增强了口腔癌细胞系的侵袭力。为了解Periostin在口腔鳞癌侵袭、血管生成和转移中的作用，我们首先采用RT-PCR方法检测了31例口腔鳞癌中Periostin mRNA的表达，采用免疫组织化学方法检测了74例口腔鳞癌中Periostin蛋白的表达。比较Periostin表达与肿瘤浸润方式、转移及血管密度的关系。Periostin mRNA和蛋白在口腔鳞癌组织中的高表达与口腔鳞癌的浸润和转移密切相关。Periostin阳性组血管密度高于Periostin阴性组。有趣的是，重组骨膜蛋白在体外以浓度依赖性方式增强毛细血管形成。总之，这些发现表明，骨膜蛋白可以促进口腔鳞癌的侵袭和血管生成，骨膜蛋白可以成为预测口腔癌患者转移的强有力的标志物。
Oral squamous-cell carcinoma (OSCC) is one of the most common types of human cancer. Typically OSCC cells show persistent invasion that frequently leads to local recurrence and distant lymphatic metastasis. We previously identified Periostin as the gene demonstrating the highest fold change expression in the invasive clone by comparing the transcriptional profile of parent OSCC cell line and a highly invasive clone. Here, we demonstrated that Periostin overexpression enhanced invasiveness in oral cancer cell lines. To know the role of Periostin in invasion, angiogenesis and metastasis in OSCC cases, we first examined the expression of Periostin mRNA in 31 OSCC cases by RT–PCR and Periostin protein in 74 OSCC cases by immunohistochemistry. Then, we compared the Periostin expression with invasion pattern, metastasis and blood vessel density. Periostin mRNA and protein overexpression were frequently found in OSCC cases and Periostin expression was well correlated with the invasion pattern and metastasis. Moreover, blood vessel density of Periostin-positive cases was higher than those of Periostin-negative cases. Interestingly, recombinant Periostin enhanced capillary formation in vitro in a concentration-dependant manner. In summary, these findings suggest that Periostin may promote invasion and angiogenesis in OSCC, and that Periostin can be a strong marker for prediction of metastasis in oral cancer patients.
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影响因子：
7.8
作者：
Elkins, T;Hortsch, M;Bieber, A J;Snow, P M;Goodman, C S
通讯作者：
Goodman, C S
DOI：
10.1111/j.1349-7006.2001.tb01174.x
发表时间：
2001-08
期刊：
Japanese journal of cancer research : Gann
影响因子：
--
作者：
Sasaki H;Lo KM;Chen LB;Auclair D;Nakashima Y;Moriyama S;Fukai I;Tam C;Loda M;Fujii Y
通讯作者：
Fujii Y
影响因子：
5.3
作者：
Shao, R;Bao, SD;Wang, XF
通讯作者：
Wang, XF
影响因子：
9.8
作者：
Bishop, E T;Bell, G T;Wheatley, D N
通讯作者：
Wheatley, D N
影响因子：
1.7
作者：
Ünal, OF;Ayhan, A;Hosal, AS
通讯作者：
Hosal, AS