99mTc-labelled multifunctional polyethylenimine-entrapped gold nanoparticles for dual mode SPECT and CT imaging
99mTc-labelled multifunctional polyethylenimine-entrapped gold nanoparticles for dual mode SPECT and CT imaging
复制标题
用于双模式 SPECT 和 CT 成像的 99mTc 标记多功能聚乙烯亚胺包埋金纳米颗粒
DOI：
10.1080/21691401.2018.1430696
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发表时间：
2018-01
影响因子：
5.8
通讯作者：
Zhao Jinhua
中科院分区：
文献类型：
--
作者：
Zhao Lingzhou;Wen Shihui;Zhu Meilin;Li Du;Xing Yan;Shen Mingwu;Shi Xiangyang;Zhao Jinhua
摘要在本研究中，我们报道了99mtc标记的聚乙烯亚胺包覆金纳米粒子（99mTc-Au-PENPs）的合成、表征和应用，用于双模式单光子发射计算机断层扫描/计算机断层扫描（SPECT/CT）成像。选择聚乙烯亚胺（PEI）为平台，与二乙烯三胺五乙酸（DTPA）和聚乙二醇单甲基醚偶联合成Au PENPs，对PEI表面剩余胺基进行乙酰化或羟基化改性，并对99mTc进行放射性标记。用不同的方法表征了不同表面基团（乙酰基或羟基）的多功能99mTc-Au-PENPs。99mTc标记前的Au PENPs在Au浓度高达100 μM时具有胶体稳定性，血液相容性和无细胞毒性。99mtc标记的Au PENPs具有高放射化学纯度，良好的稳定性和不同器官和淋巴结的SPECT/CT成像性能。利用放射性核素标记技术和pei促进的多功能纳米平台的设计策略可以扩展到开发用于精密成像的各种新型纳米探针。
Abstract In this study, we report the synthesis, characterization and utilization of 99mTc-labelled polyethylenimine-entrapped gold nanoparticles (99mTc-Au-PENPs) for dual mode single-photon emission computed tomography/computed tomography (SPECT/CT) imaging applications. Polyethylenimine (PEI) was selected as a platform to conjugate with diethylene triamine pentacetate acid (DTPA) and polyethylene glycol monomethyl ether to synthesize Au PENPs, followed by acetylation or hydroxylation modification of the remaining PEI surface amine groups and radiolabelling of 99mTc. The generated multifunctional 99mTc-Au-PENPs with different surface groups (acetyl or hydroxyl) were characterized via different methods. The Au PENPs before 99mTc labelling are colloidally stable, haemocompatibility and noncytotoxic at an Au concentration up to 100 μM. The 99mTc-labelled Au PENPs exhibit high radiochemical purity, good stability and SPECT/CT imaging performance of different organs and lymph node. The designed strategy to use the radionuclide labelling technique and PEI-facilitated versatile nanoplatform may be extended to develop various novel nanoprobes for precision imaging applications.
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影响因子：
14
作者：
Chen, Kuan-Ju;Wolahan, Stephanie M.;Wang, Hao;Hsu, Chao-Hsiung;Chang, Hsing-Wei;Durazo, Armando;Hwang, Lian-Pin;Garcia, Mitch A.;Jiang, Ziyue K.;Wu, Lily;Lin, Yung-Ya;Tseng, Hsian-Rong
通讯作者：
Tseng, Hsian-Rong
影响因子：
9.5
作者：
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通讯作者：
Sheng-yuan Zhou;Zhenkai Wu;Xiongsheng Chen;Lianshun Jia;Wei Zhu
影响因子：
14
作者：
Yamano, Seiichi;Dai, Jisen;Montclare, Jin K.
通讯作者：
Montclare, Jin K.
影响因子：
19.7
作者：
von Schulthess, GK;Steinert, HC;Hany, TF
通讯作者：
Hany, TF
用于血池和肿瘤 CT 成像的聚乙二醇化聚乙烯亚胺包载金纳米颗粒的合成和表征
影响因子：
9.5
作者：
Zhou, Benqing;Zheng, Linfeng;Shi, Xiangyang
通讯作者：
Shi, Xiangyang