Simultaneous quantification of methadone, cocaine, opiates, and metabolites in human placenta by liquid chromatography-mass spectrometry.
Simultaneous quantification of methadone, cocaine, opiates, and metabolites in human placenta by liquid chromatography-mass spectrometry.
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DOI：
10.1093/jat/33.5.243
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发表时间：
2009-06
影响因子：
2.5
通讯作者：
Huestis MA
中科院分区：
文献类型：
--
作者：
de Castro A;Concheiro M;Shakleya DM;Huestis MA
采用液相色谱-离子阱质谱法测定人胎盘中美沙酮、2-乙基-1,5-二甲基-3,3-二苯基吡咯烷、可卡因、苯甲酰ecgonine、6-乙酰吗啡、吗啡和可待因的含量。样品（1g）均质后进行固相萃取。色谱分离采用增效极性反相色谱柱，梯度为0.1%甲酸和乙腈。美沙酮在10 ~ 2000 ng/g范围内线性，其他分析物在2.5 ~ 500 ng/g范围内线性。检出限为0.25 ~ 2.5 ng/g，不精确度< 9.1%CV，分析回收率84.4 ~ 113.3%，萃取效率bb0.46%，基质效应−8.0 ~ 129.9%，工艺效率24.2 ~ 201.0%。通过对5例接受美沙酮药物治疗的阿片类药物依赖妇女胎盘标本的分析，证明了方法的适用性，分娩当天美沙酮剂量为65 ~ 95 mg。这是控制用药后胎盘中美沙酮及其代谢物浓度的首次数据。胎盘中其他常见药物滥用的检测也将提高我们对该矩阵在检测子宫内药物暴露和研究母胎双体药物处置方面的有用性的认识。
A validated method for quantifying methadone, 2-ethylidene-1,5-dimethyl-3,3-diphenylpyrrolidine, cocaine, benzoylecgonine, 6-acetylmorphine, morphine, and codeine in human placenta by liquid chromatography–ion trap mass spectrometry is described. Specimens (1 g) were homogenized and subjected to solid-phase extraction. Chromatographic separation was performed on a Synergi Polar RP column with a gradient of 0.1% formic acid and acetonitrile. The method was linear from 10 to 2000 ng/g for methadone and 2.5 to 500 ng/g for other analytes. Limits of detection were 0.25–2.5 ng/g, imprecisions < 9.1%CV, analytical recoveries 84.4–113.3%, extraction efficiencies > 46%, matrix effects −8.0–129.9%, and process efficiencies 24.2–201.0%. Method applicability was demonstrated by analysis of five placenta specimens from opioid-dependent women receiving methadone pharmacotherapy, with methadone doses ranging from 65 to 95 mg on the day of delivery. These are the first data on placenta concentrations of methadone and metabolites after controlled drug administration. Detection of other common drugs of abuse in placenta will also improve our knowledge of the usefulness of this matrix for detecting in utero drug exposure and studying disposition of drugs in the maternal-fetal dyad.
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DOI：
10.1016/s1044-0305(03)00574-9
发表时间：
2003-11-01
影响因子：
3.2
作者：
Dams, R;Huestis, MA;Murphy, CM
通讯作者：
Murphy, CM
影响因子：
3.7
作者：
Jones, Hendree E.;O'Grady, Kevin E.;Tuten, Michelle
通讯作者：
Tuten, Michelle
影响因子：
2.8
作者：
FINNEGAN, LP
通讯作者：
FINNEGAN, LP
影响因子：
7.4
作者：
Dams, R;Murphy, CM;Huestis, MA
通讯作者：
Huestis, MA
影响因子：
2
作者：
Dams, R;Murphy, CM;Huestis, MA
通讯作者：
Huestis, MA